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MilliporeSigma™ Novagen™ Origami™ 2 (DE3) Competent Cells

Catalog No. 713454 Shop All MilliporeSigma Products
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71-345-4 1 mL
71-345-3 0.4 mL
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Catalog No. 71-345-4 Supplier MilliporeSigma™ Supplier No. 713454
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Description

Origami 2 strains have mutations in glutathione reducatase (gor) and thioredoxin reductase (trxB), facilitating proper disulfide bond formation.

Origami™ 2 host strains are K-12 derivatives that have mutations in both the thioredoxin reductase (trxB) and glutathione reductase (gor) genes, which greatly enhance disulfide bond formation in the E. coli cytoplasm. The Origami 2 strains are kanamycin sensitive; like the original Origami strains, the gor mutation is still selected for by tetracycline. To reduce the possibility of disulfide bond formation between molecules, strains containing mutations in trxB and gor are recommended only for the expression of proteins that require disulfide bond formation for proper folding. DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
TRUSTED_SUSTAINABILITY

Specifications

Efficiency Guaranteed efficiency: >2 x 106 cfu/μg
Content And Storage ≤-70°C
Quantity 1 mL
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