EMD Millipore™ ReBlot Plus Kit and Reagents 

Blots can be stripped of antibodies in approximately 15 minutes at room temperature

Includes: Mild Antibody Stripping Solution (10X), 50mL; Strong Antibody Stripping Solution (10X), 50mL

Catalog Number	Mfr. No.	Product Type	Quantity	Kit Contents	Storage Requirements
2500MI	EMD Millipore™ 2500	ReBlot plus kit	Each	50mL Mild Antibody stripping solution 10X, 50mL strong Antibody stripping solution 10X	4°C
2504MI	EMD Millipore™ 2504	ReBlot Plus 10X Strong Antibody stripping solution	50mL	50mL Strong Antibody stripping solution 10X	2° to 8°C
2502MI	EMD Millipore™ 2502	ReBlot Plus 10X mild Antibody stripping solution	50mL	25mL Mild Antibody stripping solution 10X	2° to 8°C

Description

Western blotting is a commonly used technique for studying protein function and localization. Typically, protein samples are electrophoresed on SDS-PAGE and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid-based technologies, which allow reuse of Southern and Northern blots, it has been difficult to reuse Western blots.

• No pungent-smelling β-mercaptoethanol is contained in the Antibody Stripping Solution
• Antibody stripping is done at room temperature—no heating of blots is required
• Blots may be reused in 25 min.

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Specifications

• Applications: Western blotting