MilliporeSigma™ TNF-α, Human, Recombinant Protein, E. coli

Catalog No.	Quantity
65-420-510UG	10 μg

Catalog No. 65-420-510UG Supplier MilliporeSigma™ Supplier No. 65420510UG

Description

Recombinant, human tumor necrosis factor-α expressed in E. coli. A cytokine produced primarily by activated macrophages that activates a variety of immune defense mechanisms by interactions with polymorphonuclear leukocytes, T cells, antibody-producing B lymphocytes, fibroblasts, and hematopoietic bone marrow cells. Activity is not species-specific. Induces apoptosis in human blood and bone marrow neutrophils and in endothelial cells. Increases the iNOS levels in vascular smooth muscle cells. Involved in pathophysiological processes of several chronic and acute diseases. Stimulates stress activated protein kinase (SAPK).

Specifications

• Formulation: Carrier-free.
• Preparation Method: Reconstitute with sterile, dH₂O to a final concentration of 0.1-1mg/mL. Further dilutions should be made in low endotoxin medium or aqueous buffer containing carrier protein such as heat inactivated FCS or tissue culture grade BSA.
• Purification Method: SDS-PAGE
• Quantity: 10 μg
• Source: E. coli
• Immunogen: TNF-α
• Storage Requirements: Following reconstitution, aliquot and freeze (−20°C or −70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at −20°C or −70°C or up to 1 month at 4°C.
• Regulatory Status: RUO
• Species: Human
• Recombinant: Recombinant
• Form: Lyophilized
• Purity or Quality Grade: ≥95%