Invitrogen™ Endogenous Biotin-Blocking Kit

Catalog No.	Quantity
E21390	1 kit

Catalog No. E21390

Supplier: Invitrogen™ E21390

Description

Endogenous Biotin-Blocking Kit

The Endogenous Biotin-Blocking Kit provides a method and reagents for a pretreatment that reduces or eliminates background signals when biotin-avidin or biotin-streptavidin detection systems are used to identify cellular targets.

The Endogenous Biotin-Blocking Kit offers:

Improved results—increased signal-to-noise ratio facilitates cell target identification

Ease of use—pipette-free dropper bottle offers speed and convenience

Fast—endogenous background reduction in under an hour

Flexibility—the kit can be used to help improve results from conventional immunohistochemistry, immunocytochemistry, RNA FISH, and ELISA procedures

Biotin occurs naturally, functioning as an enzyme cofactor in the cytosol and mitochondria of a wide variety of cell types. The Endogenous Biotin-Blocking Kit minimizes interference from the endogenous biotin found in enzymes of mammalian cells and tissues, through a two-step blocking strategy. First, an excess of unlabeled streptavidin is added to the specimen to bind endogenous biotin-rich enzymes. The streptavidin is subsequently blocked with an excess of unlabeled biotin, effectively rendering the cell or tissue sample free of available biotin-binding sites.

Specifications

• Type: Endogenous Biotin-Blocking Kit
• Reagent Type: Blocking⁄Background Suppression Reagent
• Content And Storage: Store in refrigerator at 2°C to 8°C
• Quantity: 1 kit
• Shipping Condition: Room Temperature

For Research Use Only. Not for use in diagnostic procedures.