Invitrogen™ Endostatin Polyclonal Antibody

Catalog No.	Quantity
PA1601	100 μg

Catalog No. PA1601 Supplier Invitrogen™ Supplier No. PA1601

Description

Rabbit Polyclonal Antibody

PA1-601 detects recombinant human and mouse endostatin. PA1-601 has been successfully used in Western blot, IF/ICC and IHC (P) procedures. By Western blot, this antibody detects a 20 kDa protein representing recombinant human endostatin. PA1-601 immunizing peptides are two peptides derived from mouse and human endostatin protein corresponding to amino acid residues 129-142 of endostatin. PA1-601 can be used with blocking peptide PEP-112.

Endostatin is able to avoke non-uniform response for proliferation, cell mount and migration of entothelial cells, which different endostatin binding characteristic, leads to the assumption that endostatin effect is strongly dependent from endothelial cell type. Furthermore endostatin inhibits angiogenesis and tumor growth in vivo by inducing apoptosis in endothelial cells. The local delivery of endostatin seems to specifically affect tumor-associated microvessels by reduction of the vessel density, diameter and functionality. Tumor cell migration and invasion was greatly reduced in the endostatin treated animals. Endostatin is non-toxic and does not induce aquired drug resistance and has therefor become a potent new therapy strategy in solid neoplasias. This therapy appears to have a high potential not only for the treatment of gliomas, the most common brain tumors, but also in other tumors. The ability of endostatin to inhibit neoangiogenesis is mediated, at least in part, by Zn^+2 binding and elastase processing. Widespread endostatin expression was found in elactic fibers in vessel walls and in some other basement membrane zones. Endostatin is released by neurons to accumulate in amyloid plaques in Alzheimer's disease.

Specifications

• Antigen: Endostatin
• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene: COL18A1
• Gene Accession No.: P39060, P39061
• Gene Alias: antiangiogenic agent; Col18a1; Collagen alpha-1(XVIII) chain; collagen type XVIII alpha 1 chain; collagen, type XVIII, alpha 1; endostatin; FLJ27325; FLJ34914; KNO; KNO1; KS; MGC74745; multi-functional protein MFP; NC1; Non-collagenous domain 1; procollagen, type XVIII, alpha 1
• Gene Symbols: COL18A1
• Host Species: Rabbit
• Immunogen: Synthetic peptides corresponding to residues R(1718) R L M E S Y C E T W R T E(1731) of Mouse Endostatin and R(1699) R L T E S Y C E T W R T E(1712) of Human Endostatin.
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 12822, 80781
• Target Species: Human, Mouse
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG