Invitrogen™ Human Endothelin-1 (ET-1) ELISA Kit

Description

ELISA

The Endothelin-1 (ET-1) Multispecies ELISA quantitates Endothelin-1 in human, bovine, procine, dog, rat, and mouse serum, plasma, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Endothelin-1. Principle of the method The Endothelin-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is inversely proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene.

Specifications

• Accession Number: P05305
• Assay Range: 0.781 to 100 pg/mL
• Assay Sensitivity: 0.579 pg/mL
• Description: Endothelin-1 (ET-1) ELISA Kit
• Assay: Multiplex assay on the Luminex Platform
• Conjugate: HRP
• Detection Method: Fluorescence
• Product Type: ELISA
• Protein Family: Peptide hormone
• Sample Type: Plasma, Serum, Supernatant, Urine
• Sufficient For: 96 assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 1906
• Gene Symbol: EDN1
• Interassay CV: 3.7%
• Intraassay CV: 3.3
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, HRP-Conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Extraction Solution
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Research Discipline: Cell Signaling
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 2.5 μL, Urine 50 μL
• Storage Requirements: -20°C
• Target: Endothelin 1
• Test Time: 1 hrs 20 mins
• Total Assay Time: 2 hrs 30 mins

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov