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Bioassay Systems EnzyChrom™ Uric Acid Assay Kit II, Uric acid determination in biological samples such as cell culture media, biofluids (serum, urine), cell and tissue extracts.
Supplier: Bioassay Systems EUAC100
URIC ACID is the breakdown product of purines such as adenosine and inosine. Humans and other primates are incapable of further metabolizing uric acid. In humans, uric acid is excreted via the kidneys, but a failure to remove excess uric acid can lead to conditions such as gout or uric acid kidney stones. BioAssay Systems’ method provides a simple and high-throughput assay for measuring uric acid levels. In this assay, uric acid is enzymatically converted to allantoin, releasing H2O2. The resulting H2O2 reacts with a specific dye to form a pink-colored product. The change in OD570nm or fluorescence intensity at λex/em = 530/585nm is directly proportional to the uric acid present in the sample.
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