Bioassay Systems EnzyFluo™ Fatty Acyl-CoA Assay Kit, For quantitative determination of fatty acyl-CoAs in tissue and cell lysates.

Supplier:  Bioassay Systems EFCOA100

Fatty Acyl-CoAs are a combination of Coenzyme A covalently bound to long-chain fatty acids. Fatty acyl-CoAs serve as both a precursor for triglyceride and phospholipids synthesis and a product of lipid catabolism. Catabolically, they facilitate the transport of fatty acids to the mitochondria for shuttling via the carnitine shuttle and subsequent β-oxidation of the fatty acid. Conversion of fatty acids to fatty acyl-CoAs is necessary for the generation of ATP during periods of high energy demand. Elevated fatty acyl-CoA levels may indicate dysregulation of lipid metabolism such as due to insulin resistance. Certain genetic disorders may also affect fatty acyl-CoA levels. BioAssay Systems’ fluorimetric fatty acyl-CoA assay works using a combination of enzymes that utilize fatty acyl-CoA as a substrate, which is coupled to form a fluorescent product. The resulting fluorescence intensity at λexc/em = 530/585 nm is linear to the fatty acyl-CoA concentration in the sample.

Catalog No. 50-001-44369