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Bioassay Systems EnzyFluo™ Uricase Assay Kit, Uricase activity in biological samples such as cell culture, non-human biofluids, cell extracts, and plant tissue extracts.
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Supplier:  Bioassay Systems EURIC100

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URICASE or URATE OXIDASE (E.C. 1.7.3.3) initiates the oxidation of uric acid, eventually yielding allantoin. Uricase activity is found throughout plants, animals, and bacteria, but it is not found in hominids. As a result, humans develop gout when the levels of uric acid become too high. BioAssay Systems’ method provides a simple and high-throughput assay for measuring uricase enzyme activity. In this assay, uric acid is enzymatically converted to allantoin, releasing H2O2. The resulting H2O2 reacts with a specific dye to form a pink-colored product. The change in fluorescence intensity at 530/585 nm is directly proportional to the uricase activity in the sample.

Catalog No. 50-001-44375


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