Invitrogen™ Eos Monoclonal Antibody (ESB7C2), PE, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Supplier:  Invitrogen™ 12575880

Catalog No. 12-575-880

Description

Description: This ESB7C2 monoclonal antibody reacts with mouse Eos (IKZF4, ZNFN1A4), a zinc finger transcription factor and member of the Ikaros family. Among the Ikaros family of proteins, Eos has the highest homology with Helios. Eos can homodimerize with itself and form heterodimers with Ikaros. Eos was first identified in the nervous system and was cloned from astrocytes. Later, Eos was found to be involved in the regulation of osteoclast differentiation. Eos has also been reported to be involved in negatively regulating gene expression in Foxp3-expressing regulatory T cells in mice. Applications Reported: This ESB7C2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This ESB7C2 antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the using the Foxp3/Transcription Factor Buffer Set (cat. 00-5523) and protocol. Slight modifications in the protocol using this antibody have been demonstrated to improve the staining resolution. We recommend the staining time of minimum 60 minutes and additional washes after the fixation (3 times) and staining steps (twice). This antibody can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Serine protease that has amidolytic activity, cleaving its substrates before Arg residues.

Specifications

• Antigen: Eos
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q80WM7
• Gene Symbols: Prss33
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 353130
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: ESB7C2
• Conjugate: PE
• Gene: Prss33
• Gene Alias: Eos; mT6; protease, serine 33; protease, serine, 33; Prss33; Serine protease 33; serine protease EOS; tryptase 6; Tryptase-6
• Host Species: Rat
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ

For Research Use Only.