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Novus Biologicals™ EpCAM/TROP1 Antibody Pair [HRP]
SDP

Catalog No. NB010063 Shop All R&D Systems Products
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NB010063 15 Plates
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Catalog No. NB010063 Supplier Novus Biologicals™ Supplier No. NBP27951215PLATES
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Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human EpCAM/TROP1.

Specifications

Assay Range 15.625 pg/mL
Assay Sensitivity 15.625-1000 pg/mL
Assay Sandwich ELISA
Conjugate HRP
Product Type Antibody Pairs
Specificity This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human EpCAM/TROP1.
Target Species Human
For Use With (Application) Sandwich ELISA
Gene Alias 17-1A, 323/A3, ACSTD1, antigen identified by monoclonal AUA1, CD326 antigen, Cell surface glycoprotein Trop-1, chromosome 4, surface marker (35kD glycoprotein), DIAR5, EGP, EGP-2, EGP314, EGP40, EpCAM, epithelial cell adhesion molecule, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, ESA, GA733-2EGP34, hEGP314, HNPCC8, KS 1/4 antigen, KS1/4, KSAHEA125, M1S2, M4S1Ly74, Major gastrointestinal tumor-associated protein GA733-2, MIC18MH99, MOC31, TACST-1, TACSTD1, TROP1CD326, Tumor-associated calcium signal transducer 1CO-17A
Gene ID (Entrez) 4072
Gene Symbol EPCAM
Host Species Rabbit/Mouse
Kit Contents
  • Standard: Each vial contains 180 ng of recombinant Human EpCAM/TROP1.
  • Capture Antibody: 0.5 mg/mL of mouse anti-Human EpCAM/TROP1 monoclonal antibody (in PBS, pH 7.4).
  • Detection Antibody: 0.2 mg/mL of rabbit anti-Human EpCAM/TROP1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  • Quantity 15 Plates
    Regulatory Status RUO
    Research Discipline Cancer, Cancer Stem Cells
    Storage Requirements Storage is content dependent.
    Target EpCAM/TROP1
    Technique 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended.
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