Novus Biologicals™ EpCAM/TROP1 Antibody Pair [HRP]

Description

Rabbit Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Mouse EpCAM/TROP1.

Specifications

• Assay Range: 78 pg/mL
• Assay Sensitivity: 78.13-5000 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Mouse EpCAM/TROP1.
• Target Species: Mouse
• For Use With (Application): Sandwich ELISA
• Gene Alias: 17-1A, 323/A3, ACSTD1, antigen identified by monoclonal AUA1, CD326 antigen, Cell surface glycoprotein Trop-1, chromosome 4, surface marker (35kD glycoprotein), DIAR5, EGP, EGP-2, EGP314, EGP40, EpCAM, epithelial cell adhesion molecule, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, ESA, GA733-2EGP34, hEGP314, HNPCC8, KS 1/4 antigen, KS1/4, KSAHEA125, M1S2, M4S1Ly74, Major gastrointestinal tumor-associated protein GA733-2, MIC18MH99, MOC31, TACST-1, TACSTD1, TROP1CD326, Tumor-associated calcium signal transducer 1CO-17A
• Gene ID (Entrez): 4072
• Gene Symbol: EPCAM
• Host Species: Rabbit
• Kit Contents:
  Standard: Each vial contains 400 ng of recombinant mouse EpCAM/TROP1.
  Capture Antibody: 1.0 mg/mL of rabbit anti-mouse EpCAM/TROP1 monoclonal antibody (in PBS, pH 7.4).
  Detection Antibody: 0.5 mg/mL rabbit anti-EpCAM/TROP1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % glycerol, pH 7.4).
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Cancer, Cancer Stem Cells
• Storage Requirements: Storage is content dependent.
• Target: EpCAM/TROP1
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in CBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 5 ng/mL is recommended.