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Invitrogen™ Epo Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Catalog No. 500P318BT50
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500 μg
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Catalog No. Quantity
500P318BT50 50 μg
500P318BT25 25 μg
50-271-8516 500 μg
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Catalog No. 500P318BT50 Supplier Invitrogen™ Supplier No. 500P318BT50UG
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Description

Rabbit Polyclonal Antibody

AA Sequence of recombinant protein: APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human EPO. Anti-Human EPO-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human EPO by sandwich ELISA (using 100 μL/well), a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human EPO (500-P318) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human EPO. Western Blot: To detect Human EPO by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human EPO is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P318BT-1MG will be provided as 2 x 500 μg

Erythropoietin (EPO) protein is a growth factor essential for the survival, proliferation, and differentiation of erythroid precursors. EPO is not responsible for early commitment to the lineage, and instead induces the final maturation of partially differentiated erythroid cells and the production of red cell-specific proteins, such as hemoglobin. The EPO receptor (EPOR) is present in higher levels on precursor cells, and expression diminishes with cell maturation.Hepatocytes are the main source of EPO in the fetus, followed by interstitial cells of the kidney after birth and through adulthood. EPO expression is upregulated during hypoxia, and low levels of transcription have also been detected in lung and brain tissue under these conditions. Recent data suggest that EPO may also play a role in the protection of neuronal tissue from damage and initiation of repair.
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Specifications

Antigen Epo
Applications ELISA, Western Blot
Classification Polyclonal
Concentration 0.1-1.0 mg/mL
Conjugate Biotin
Formulation PBS with no preservative
Gene EPO
Gene Accession No. P01588
Gene Alias EP; EPO; Epoetin; erythropoietin; MGC138142; MVCD2
Gene Symbols EPO
Host Species Rabbit
Immunogen CHO cells-derived Recombinant Human EPO.
Purification Method Antigen affinity chromatography
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 2056
Target Species Human
Content And Storage -20°C
Product Type Antibody
Form Lyophilized
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