Epo Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Pepro Tech Inc 500P318BT50UG

Catalog No. 500P318BT50

Description

AA Sequence of recombinant protein: APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human EPO. Anti-Human EPO-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human EPO by sandwich ELISA (using 100 μL/well), a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human EPO (500-P318) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human EPO. Western Blot: To detect Human EPO by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human EPO is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Epo Polyclonal antibody specifically detects Epo in Human samples. It is validated for ELISA, Western Blot

Specifications

• Antigen: Epo
• Classification: Polyclonal
• Formulation: PBS with no preservative
• Gene Accession No.: P01588
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 2056
• Content And Storage: -20°C

• Applications: ELISA, Western Blot
• Conjugate: Biotin
• Gene: EPO
• Gene Alias: EP; EPO; Epoetin; erythropoietin; MGC138142; MVCD2
• Immunogen: CHO cells-derived Recombinant Human EPO
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Human
• Form: Lyophilized