Epo Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
500P31850UG	Invitrogen™ 500P31850UG	50 μg
500P318100	Invitrogen™ 500P318100UG	100 μg
500P3181MG	Invitrogen™ 500P3181MG	1 mg

Description

AA Sequence of recombinant protein: APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human EPO. Anti-Human EPO-specific antibody was purified by affinity chromatography employing an immobilized Human EPO matrix. Sandwich ELISA: To detect Human EPO by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human EPO (500-P318Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human EPO. Western Blot: To detect Human EPO by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human EPO is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Erythropoietin (EPO) protein is a growth factor essential for the survival, proliferation, and differentiation of erythroid precursors. EPO is not responsible for early commitment to the lineage, and instead induces the final maturation of partially differentiated erythroid cells and the production of red cell-specific proteins, such as hemoglobin. The EPO receptor (EPOR) is present in higher levels on precursor cells, and expression diminishes with cell maturation.Hepatocytes are the main source of EPO in the fetus, followed by interstitial cells of the kidney after birth and through adulthood. EPO expression is upregulated during hypoxia, and low levels of transcription have also been detected in lung and brain tissue under these conditions. Recent data suggest that EPO may also play a role in the protection of neuronal tissue from damage and initiation of repair.

Specifications

• Antigen: Epo
• Applications: ELISA, Western Blot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Target Species: Human
• Gene Accession No.: P01588
• Gene ID (Entrez): 2056
• Immunogen: CHO cells-derived Recombinant Human EPO.
• Purification Method: Antigen Affinity Chromatography
• Gene: EPO

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene Alias: EP; EPO; Epoetin; erythropoietin; MGC138142; MVCD2
• Gene Symbols: EPO
• Primary or Secondary: Primary
• Content And Storage: -20°C