Invitrogen™ Estrogen Receptor alpha Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA1309

Catalog No. PA1309

Description

PA1-309 detects estrogen receptor (ER) alpha from human, porcine and rat tissues and cells. PA1-309 has been successfully used in Western blot, immunofluoresence and immunohistochemistry procedures. By Western blot, this antibody detects an ~64 kDa protein representing ER alpha from MCF-7 cell extracts. Immunohistochemical staining of ER alpha in rat prostate with PA1-309 results in primarily nuclear staining. The PA1-309 immunogen is a synthetic peptide corresponding to residues N(21) E L E P L N R P Q L K(32) C of human ER alpha. This peptide sequence is completely conserved between human, rat, mouse, rabbit, sheep, porcine and bovine species. PA1-309 immunizing peptide (Cat. # PEP-037) is available for use in neutralization and control experiments. Recombinant ER alpha expressed in baculovirus system (Cat. # RP-310) can be purchased for control experiments in Western blot and gel shift assays.

Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.

Specifications

• Antigen: Estrogen Receptor alpha
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ESR1
• Gene Alias: DKFZp686N23123; ER; Er alpha; ER36; ERa; ERalpha; ER-alpha; ERalpha protein; ESR; esr 1; Esr1; esr-1; ESRA; Estr; Estra; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor 1; estrogen receptor 1 (alpha); estrogen receptor alpha; estrogen receptor alpha c-terminus splice variant 1-2; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha splice variant, CTERP-1; estrogen receptor alpha splice variant, ERalphaDup5; estrogen receptor alpha splice variant, ERalphai45a; estrogen receptor alpha splice variant, ERalphai45bL; estrogen receptor alpha splice variant, ERalphai45bS; estrogen receptor alpha splice variant, ERalphai45c; estrogen receptor alpha splice variant, ERalphai56; estrogen receptor alpha splice variant, ERalphai67; estrogen receptor alpha variant delta 4; estrogen receptor protein; estrogen receptor, alpha; ESTRR; hER-alpha36; I79_001166; Nr3a1; nuclear receptor; nuclear receptor subfamily 3 group A member 1; RNESTROR; RP1-130E4.1; unnamed protein product
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 2099, 24890, 397435
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: P03372, P06211, Q29040
• Gene Symbols: ESR1
• Immunogen: Synthetic peptide corresponding to residues N(21) E L E P L N R P Q L K(32) C of human ER alpha.
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Human, Rat, Pig
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov