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Invitrogen™ EVOS™ M5000 Imaging System
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Catalog No. 12563631
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Catalog No. 12-563-631 Supplier Invitrogen™ Supplier No. AMF5000

Item Discontinued This product is not available for sale online. Please contact Customer Service at 1-800-766-7000 or www.fishersci.com/cellanalysisdemo to request a demonstration customized to your application.

Includes

EVOS™ M5000 Imaging System, EVOS™ M5000 Accessory Kit, EVOS™ M5000 Collateral Pack

The Invitrogen™ EVOS™ M5000 Cell Imaging System brings simple, automated microscopy within reach. All-in-one design includes high resolution fluorescence imaging, unique color illumination, a full suite of fluorescent filters, and intuitive software.

The EVOS M5000 Imaging System offers you these important advantages:

  • Easy installation—no maintenance, assembly, alignment, or calibration
  • User-defined four-color fluorescence, transmitted light, and color applications
  • Autofocus, Z-stack capability, time-lapse imaging, and single-click multi-channel capture
  • On-board software for acquisition, analysis, and automated cell counting

Fully integrated imaging system

The EVOS M5000 microscope combines precision components with a unique modern design functionality that enables high-quality fluorescence and color imaging anywhere with unprecedented flexibility. It is a fully integrated imaging system that combines precision optics, an 18.5” high-resolution articulated LCD monitor, and a highly sensitive 3.2 MP monochrome CMOS camera (2048 x 1536) with 3.45-μm pixel resolution. The monochrome camera affords the best sensitivity for detection of faint fluorescence signals and allows quantitative analysis. At the same time the condenser includes a novel LED-based RGB illumination scheme that enables high-quality, low-noise color image acquisition using the same monochrome sensor, enabling rendering of true color in transmitted light, which is beneficial when imaging stained tissue samples such as H&E stained tissue sections.

Versatile and highly configurable
With a five-position objective turret and the ability to acquire images in four fluorescence channels, transmitted light phase-contrast, and color, the optical system can be easily configured to meet your needs. Choose from our broad range of high-quality long-working-distance and coverslip-corrected objectives for use with plastic vessels or glass sides. Available from 1.25X to 100X magnification with achromat, fluorite, or apochromat correction, together with over twenty LED light cubes, the sample imaging options are limitless. Interchangeable vessel holders accommodate most vessel types and sizes, including slides, multi-well plates, culture flasks, and Petri dishes, and afford precise control and sample alignment by the automated stage.

Easy to use and reliable
The EVOS M5000 Imaging System is powerful, yet offers the simplicity of plug-and-play. It requires no warm-up or cool-down periods, allowing you to capture images in minutes. The environmentally safe, mercury-free LED lights are rated for 50,000 hours and afford exceptional stability and durability—you can turn the unit on and off whenever you need to image a sample. The long lifetime and low energy consumption translate into significantly lower operating costs compared to instruments with conventional light sources. Designed by biologists for biologists with a modern, icon-based interface, the EVOS M5000 microscope is remarkably easy to use.

Compliance

For Research Use Only. Not for use in diagnostic procedures.

Warranty and Services

One year of warranty (standard warranty) is included with your instrument purchase.

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

TRUSTED_SUSTAINABILITY

Specifications

Type Cell Imaging System
Product Line EVOS
Camera 3.2 MP Monochrome CMOS
For Use With (Application) Imaging
For Use With (Equipment) EVOS™
Color Assorted Colors
Model EVOS M5000
Light Source LED
Magnification 1.25X to 100X
Objectives 5 Position Turret
Resolution 1920 x 1080 pixels
Display 18.5 in. Articulated LCD
Dimensions 18 in. (W) x 23 in. (H) x 18 in. (D)
Weight (English) 36 lb.
Content And Storage EVOS M5000 Imaging System
• 18.5in articulated LCD color monitor, 1920 x 1080 pixel resolution
• Embedded PC with 4 GB RAM
• EPI Axis Shipping Restraint Block
• Cube Access Tool
• Light Cube Blank
• Stage Quick Release Pin
• EVOS Condenser Light Shield

EVOS M5000 Accessory Kit
• USB WiFi Dongle
• USB Wireless Keyboard
• Vessel Holder for Slides (AMEPVH001)
• Universal Vessel Holder (AMEPVH009)
• EVOS Calibration Slide (AMEP4720)
• Universal Power Supply, 12V, 5A
• Power Cord, Type B (North America)
• UV Shield
• EVOS Light Shield
• Block Condenser Slider (AMEP4688)
• Condenser Slider for 4X Objectives (AMEP4738)
• Diffusion Condenser Slider for Brightfield Applications (AMEPDFS1)
• EVOS Dust Cover
• Objective Case with Adjustable Compartments
• Hex Driver, 2 mm

EVOS M5000 Collateral Pack
• EVOS M5000 Quick Start Guide
• EVOS M5000 Smart Start Module Flyer
• 16 GB USB 3.0 Drive with Manual and Accessory Documentation
• Mouse Pad
• Tech Support Card

* Note that one (1) country-specific power cord must be ordered separately in regions not using the Type B (North America) power plug:
AMEP4646 EVOS Power Cord, Type C (Europe)
AMEP4708 EVOS Power Cord, Type D (India/South Africa)
AMEP4645 EVOS Power Cord, Type G (United Kingdom)
AMEP4649 EVOS Power Cord, Type H (Israel)
AMEP4647 EVOS Power Cord, Type I (Australia)
AMEP4782 Power Cord, Type I (China, CCC certified)
AMEP4786 Power Cord Type J (Switzerland)
AMEP4648 EVOS Power Cord, Type L (Italy)

Quantity 1 system
Shipping Condition Room Temperature
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Can I visualize Alexa Fluor 488 dye using the EVOS M5000 Imaging System?

Yes, you can visualize Alexa Fluor 488 dye using the EVOS M5000 Imaging System. The system supports the GFP light cube, which is compatible with Alexa Fluor 488 dye. This allows for effective imaging of samples stained with Alexa Fluor 488 dye.

For more details on compatible dyes and light cubes, please refer to page 136 of the EVOS M5000 Imaging System User Guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017563_EVOS_M5000_Imaging_System_UG.pdf).

What laser should I use with the EVOS M5000 Imaging System?

The EVOS M5000 Imaging System does not use lasers for fluorescence imaging. Instead, it utilizes LED light cubes. For more details on compatible dyes and light cubes, please refer to page 136 of the EVOS M5000 Imaging System User Guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017563_EVOS_M5000_Imaging_System_UG.pdf).

Can I visualize Alexa Fluor 647 dye using the EVOS M5000 Imaging System?

Yes, you can visualize Alexa Fluor 647 dye using the EVOS M5000 Imaging System. The system supports the Cy5 light cube, which is compatible with Alexa Fluor 647 dye. This allows for effective imaging of samples stained with Alexa Fluor 647 dye.

For more details on compatible dyes and light cubes, please refer to page 136 of the EVOS M5000 Imaging System User Guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017563_EVOS_M5000_Imaging_System_UG.pdf).

Can I upgrade my EVOS M5000 Imaging System (Cat. No. AMF5000)?

Yes, you can add different objectives, wavelength LEDs, vessel holders, OnStage Incubation (temp/humidity/CO2/hypoxic), even image analysis software (Celleste) at any time. If needed, we also offer an upgrade path to the fully automated EVOS M7000 Imaging System as well.
Please contact Technical Support at tech.support@thermofisher.com for more information.

I'm imaging my fluorescently stained samples on EVOS M7000/ EVOS M5000 imaging system and I can't see any color on the displayed image. How do I visualize my images in color?

Toggle image color display provides an option to display images in pseudo-color or in grayscale in the Viewing area. By default, pseudo-color display is on. Click the Image color display button to display the sample pseudo-colored in the default emission color of the selected channels. Click again for grayscale display.

To change the default pseudo-color, you can go to Settings Tab-> Filter cubes and adjust the pseudo-color for a selected channel to the desired color.

What service options do you offer for the EVOS M5000 Imaging System?

The EVOS M5000 Imaging System comes with a standard 12-month warranty that allows for the unit replacement in case of malfunction. Additional 1-year extended warranty can be purchased separately (contact Technical Support at techsupport@thermofisher.com for details), or alternatively, one can purchase the EVOS M5000 Imaging System Extended Warranty Package (Cat. No. A40486) that will provide the EVOS M5000 Imaging System with the standard 12-month warranty plus 1-year extended warranty.

How should I maintain the EVOS M5000 Imaging System?

For regular maintenance, the EVOS M5000 Imaging System can be turned off, wiped gently with lightly dampened Kimwipes™ tissues, and covered with the dust cover provided with the instrument.

For decontamination, lightly wipe working surfaces of the EVOS M5000 Imaging System (stage top, objective turret, housing, etc.) with paper towels or Kimwipes™ tissues dampened with 70% ethanol or 4,000 ppm hydrogen peroxide (H2O2).

What is the limit of resolution for the EVOS M5000 Imaging System?

At the highest magnification (100X), the field of view is 126 µm x 95 µm with an EVOS 100X objective and 103 µm x 78 µm with an Olympus 100X objective. The internal magnification is 22.8X for EVOS objectives and 27.9X for Olympus objectives.

What applications can I use the EVOS M5000 Imaging System for?

The EVOS M5000 Imaging System is a fully integrated digital-inverted benchtop microscope for four-color fluorescence, transmitted light, and color imaging. Compatible applications include fluorescent cell and tissue staining, fluorescence viability assays, fluorescence reporter assays, apoptosis detection, transduction efficiency, cell counting and confluence, Z-Stack, time-lapse, and phase contrast.

How do the specific parameters on an objective relate to my application and how do I select the right one for my EVOS imaging system?

Please find a comprehensive objective selection guide in the link below. It provides detailed guidance based on sample type, vessel type and mode of imaging.
https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/cell-imaging-systems/evos-objectives/selection-guide-evos-objectives.html


For Research Use Only. Not for use in diagnostic procedures.

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