Invitrogen™ EVOS™ XL Core Imaging System

Catalog No.	Quantity
12-562-751	1 each

Catalog No. 12-562-751 Supplier Invitrogen™ Supplier No. AMEX1000

Description

Includes

EVOS XL Core Imaging System with fixed stage, Glass stage plate, Wireless mouse, Power adapter, Cord, Universal power plug adapters, USB flash drive (includes User Guide and Quick Start Guide), Dust cover, 3mm hex driver L-shaped key, Mechanical stage instructions

Digital, transmitted light, inverted imaging system for cell and tissue culture applications and routine cell maintenance.

Color camera and high-quality optical system deliver high-definition images with exceptional ease. With a small footprint and an ergonomic design, the all-in-one EVOS XL system is ideal for basic imaging needs and a perfect addition to any cell culture room or facility. Integrated transmitted-light inverted imaging system combines high-quality optics, a high-resolution LCD display, and a digital color camera. Images are seamlessly acquired through the user interface using a mouse and the integrated software that includes a variety of features, such as color temperature control. 

• Easy installation; no maintenance, assembly, alignment, or calibration
• Fits and operates inside a cell culture hood
• All-in-one design: digital camera, precision optics, LCD display, and USB storage
• All images can be saved onto the USB device in JPEG, BMP, or TIFF formats
• Four-position objective turret can be configured to best meet your needs using a full range of long working distance (LWD) and coverslip-corrected objectives
• LWD objectives are available from 4X to 40X; the 2X objective allows viewing of the entire well of a 96-well plate
• Compact footprint makes it easy to use the system wherever needed; entire system fits in a cell culture hood or glove box
• Universal stage provides maximum flexibility for slides, culture dishes, and flasks
• Optional mechanical stage affords more precise handling and positioning of multiwell plate vessels
• Easy plug-and-play system with simple set-up and installation; requires no warm-up or cool-down periods
• LED light source offers exceptional stability and durability; turn unit on and off whenever you need to image a sample
• Long life and low energy consumption result in lower operating costs compared to instruments with conventional light sources
• Advanced ergonomic design removes the strain associated with conventional microscopes, enables shared viewing, and makes moving the units easy

Specifications

• Optics: infinity-corrected optical system, RMS-threaded objectives with 45mm parfocal distance
• Objectives (not included): wide selection of high-quality LWD and coverslip-corrected objectives
• Illumination: adjustable intensity LED (>50,000-hr. life per light cube)
• Contrast methods: transmitted light (brightfield and phase contrast)
• Objective turret: 4-position, manual control
• Condenser: 3-position turret for brightfield and phase contrast
• Condenser working distance: 60mm
• Stage: fixed stage
• Focus mechanism: coaxial focus knobs with tension control
• Course focus: 38mm/rev
• Fine focus: 0.2mm/rev, precision 0.002mm
• LCD display: 12.1 in. high-resolution (1024 x 768 pixels) color monitor with adjustable tilt
• Camera: Color, 1/2 in. 2048 x 1536 pixels, 3.1 megapixels
• Image acquisition: embedded operating system with software for image capture and saving via mouse or front-mounted manual buttons
• Captured images: color camera; 24-bit full color; TIFF, PNG, jpeg, bmp; 2048 x 1536 pixels
• Output ports: Two USB 2.0 ports
• Power supply: AC adapter; input 100-240V, 47-63 Hz; output 12 VDC/2.0A 24W max
• Dimensions: 40.6 x 31.8 x 53.3cm (6 x 12.5 x 21 in. )
• Weight: 9.1kg (20.1 lb.) 

For Research Use Only. Not for use in diagnostic procedures.

Note: Objectives are not included; sold separately. Available objectives include: 2X (AMEP4931), 4X (AMEP4932), 10X (AMEP4933), 20X (AMEP4934), 40X (AMEP4935). For a complete EVOS XL Core microscope package with objectives included, see AMEX1100. 

Basic imaging of live and fixed cells, tissue culture determinations (confluence, density, and growth), stem cell isolation, and analysis of stained tissue slices.

Specifications

• Type: Cell Imaging System
• Product Line: EVOS
• Camera: 3.1 MP Color
• For Use With (Application): Cell Culture, Tissue Culture
• For Use With (Equipment): Brightfield Microscope
• Color: Transmitted Light
• Contrast Methods: Transmitted Light (brightfield and phase contrast)
• Format: Specific Holder Attachments
• Model: EVOS XL Core
• Light Source: LED
• Objectives: 4 Position Turret, Manual Control
• Resolution: 1024 x 768 pixels
• Stage: Universal fixed (optional mechanical stage available)
• Data Outputs: JPG Files, PNG Files, BMP Files, TIFF Files
• Display: Adjustable Tilt, 12 in. Color
• Dimensions: 13 in. (W) x 21 in. (H) x 16 in. (D)
• Weight (Metric): 9.1 kg
• Content And Storage: Unit includes:
  
  • EVOS XL Core Imaging System
  • Polycarbonate stage plate
  • Glass stage plate
  • Wireless mouse
  • Power adaptor, cord, and universal power plug adapters
  • USB flash drive (includes User Guide and Quick Start Guide)
  • Dust cover
  • 3mm hex driver L-shaped key
  
  
• Quantity: 1 each
• Frequency: 50/60 Hz
• Voltage: 100/240 VAC

Frequently Asked Questions (FAQs)

What are the dimensions of the EVOS XL Core Configured Cell Imager (Cat. No. AMEX1100)?

The following are the dimensions for the EVOS XL Core Configured Cell Imager:
- Operating height: 53.3 cm (21.0 in)
- Storage/transport height: 36.8 cm (14.5 in)
- Depth: 40.6 cm (16.0 in)
- Width: 31.8 cm (12.5 in)
However, the monitor can fold down flat, which will reduce the height to 13.25 inches, for the purpose of more easily putting it into or taking it out of a tissue culture hood.
The widest portion (in the numbers above) is the width of the monitor. The actual footprint of the base is narrower, at 8.5 inches wide (at the back of the base). The depth of the base is 16.0 in.
These dimensions are the same for all three catalog numbers (AMEX1000, AMEX1100, AMEX1200) since the base unit is the same for all three and they differ only in the objectives that come with it or, for AMEX1200, the optional mechanical stage addition.

The coarse focus knob on my EVOS XL Core Configured Cell Imager (Cat. No. AMEX1000) keeps moving/won't stay in place and is causing the image to be out of focus. How can I resolve this?

There is a tension control focus knob, located behind the right-hand course focus knob. You may just need to tighten the tension control.
If the issue persists after adjusting the tension knob, then it is possibly a hardware failure. We do not currently offer repair service for the EVOS XL core microscope, whether on-site or by mailing to us. If you believe this is a hardware failure, please contact Technical Support at tech.support@thermofisher.com for next steps and options.

How do the specific parameters on an objective relate to my application and how do I select the right one for my EVOS imaging system?

Please find a comprehensive objective selection guide in the link below. It provides detailed guidance based on sample type, vessel type and mode of imaging.
https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/cell-imaging-systems/evos-objectives/selection-guide-evos-objectives.html

How do I add a scale bar to my image on the EVOS XL Core Imaging System?

The EVOS XL Core Imaging System does not have an integrated scale bar function, but users can easily add a scale bar using the image analysis freeware ImageJ or other image analysis software.

What types of image files can I save on the EVOS XL Core Imaging System?

Image files supported by the EVOS XL Core Imaging System are: BMP, JPEG, and TIFF.

What is the EVOS XL Core Attachable Mechanical Stage for?

The EVOS XL Core Attachable Mechanical Stage (Cat. No. AMEP4712) is an optional feature, that can be purchased separately. It is an alternative to the universal, fixed stage provided with the EVOS XL Core Imaging System and allows for easier manipulation of sample vessels and slides. This mechanical stage has X-Y axis controls and a vessel holder framework that accommodates our broad range of vessel holders for smaller sample vessel types, such as microscope slides and 35 mm dishes. Larger sample vessels, such as T-75 flasks or multi-well plates, fit directly onto the stage.

What are the abilities of EVOS XL Core Imaging System? What applications can I use it for?

The EVOS XL Core Imaging System is suitable for all manual brightfield imaging applications. This system is not automated, therefore any static applications, such as cell culture visualization, manual cell counting, brightfield viability, and other manually performed experiments can be performed.

The EVOS XL Core Imaging System is fully manual and hence not capable of automated time-lapse, Z-stack or high throughput/ automated scanning imaging. For these applications, we recommend using the EVOS M7000 Imaging System.

I'm trying to acquire an image on the EVOS imaging system, but nothing shows up on the monitor. What can be causing this?

For the EVOS imaging systems:

Make certain the light is on (easy way to test this: place a piece of thin paper on the stage).
Make certain the sample is not too opaque; compare with a calibration slide or another, thinner or single-cell sample on a slide.
Check the objectives to make certain the turret is in alignment and the objective is completely threaded in its slot.
For the EVOS FL Imaging System: change the position of the light cubes.
For the EVOS FL Auto Imaging System: check the insertion of all USB ports for connectively from scope to computer.
For brightfield settings, check the condenser slider slot; make certain the condenser sliders are completely in place.

I'm using an EVOS imaging system and my objective is rubbing up against the edge of the vessel holder of my stage. How can I correct this?

Objectives can hit the vessel holder when they are focused too high in the Z axis (up and down). This is a particularly a problem with the EVOS FL Auto Imaging System during instrument start-up, when the stage moves during system initiation, or when changing objectives. Coverslip-corrected objectives tend to be wider and flatter at the top of the barrel, which means that they are more likely to run into the edges of the vessel holder, particularly if you are imaging at the edges of the sample container. In those cases, use of that objective for those areas of the container may not be possible. If the objective if “jammed” by the vessel holder, then carefully unscrew the thumbscrews of the vessel holder and lift it straight off the stage, then move the objective downward in focus and toward the center of the stage. It is a good idea to have a shut-down procedure in your lab that includes moving the objectives to the lowest magnification and focusing downward with course focus prior to turning off the instrument for the day.
An objective can be damaged by scraping against the vessel holder. If this happens, take out the objective and examine it carefully for damage, particularly on the lens.

I want to check to make sure I have the most up-to-date software for my EVOS imaging system. Where do I go for this?

Here is a page you can go to - https://www.thermofisher.com/us/en/home/products-and-services/services/instrument-qualification-services/instruments-and-services-portal/instrument-software-downloads.html. Look under the “Cell Imaging Systems” section and follow the link for your EVOS imaging system. There you will find the free download link as well as instructions to follow. We recommend you check for updates at least every six months, or if your system seems to have a software glitch of any sort.

Can the EVOS cell imaging systems be used to automatically count cells?

Only the EVOS FL Auto Imaging System and EVOS FL Auto 2 Imaging System have an automatic cell counting function. On the EVOS FL Auto Imaging System, automatic cell counting is enabled via built-in instrument firmware whereas on the EVOS FL Auto 2 Imaging System, the Celleste Image Analysis Software needs to be purchased separately to enable automatic cell counting.

The EVOS XL, EVOS FL, and EVOS FL Color imaging systems provide a manual cell counting tool that allows tagging of up to six different labels on the screen image.

What is the difference between the FLoid Cell Imaging Station and the EVOS FL imaging systems?

The FLoid Cell Imaging Station is a simple, easy-to-use transmitted-light and three-color fluorescence microscope. The three fluorescent colors, blue, green, and visible red (Texas Red dye) are set and cannot be changed to other filter sets. The magnification is also set with a 20x objective; this objective cannot be removed or changed.

On the other hand, the EVOS FL Imaging Systems are easy-to-use transmitted-light and fluorescence microscopes that allow the user access to the objective turret and light cube tray to permit multiple options for magnification and fluorescence detection. The EVOS FL Imaging Systems allow the use of 23 different light cubes and 18 different objectives (from 2x to 100x).

Can the light cubes used in the EVOS FL imaging systems also be used in the Countess II FL Automated Cell Counter?

Yes, the Countess II FL instrument uses the same light cubes as the EVOS imaging systems. The Countess II instrument does not use light cubes.

I need a new microscope system. I see that you offer a number of EVOS systems. How do I choose?

You should choose based upon the complexity of your labs' analyses and whether you need transmitted (color) camera imaging or fluorescent (monochrome) camera imaging, or both. At routine/basic analyses, we offer the EVOS XL Core Imaging System for simple transmitted light imaging (typically used in cell culture facilities) or the FLoid Cell Imaging Station for dedicated three-color fluorescence imaging. The EVOS XL Imaging System differs from the EVOS XL Core Imaging System in that it has a larger monitor, a five objective turret (only four objectives with the EVOS XL Core instrument), can be networked, and can perform cell counting.

If you need more options for fluorescent wavelengths, cell counting, or time-lapse imaging, you may consider the EVOS FL Imaging System or the EVOS FL Auto Imaging System. The EVOS FL Auto Imaging System is our most versatile option, featuring automated imaging and more. It has both color and monochrome camera options, and can even be paired with our EVOS Onstage Incubator system.

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.