Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Enzymes and Inhibitors
  4. Modifying Enzymes
  5. Thermo Scientific™ Exonuclease I (20 U/μL) 

Thermo Scientific™ Exonuclease I (20 U/μL)
GSA_VA

Catalog No. FEREN0581
Change view
Click to view available options
Quantity:
20,000 units
4,000 units
2 product options available for selection
Product selection table with 2 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
FEREN0581 4,000 units
FEREN0582 20,000 units
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 2 options available.
2 options
Catalog No. FEREN0581 Supplier Thermo Scientific™ Supplier No. EN0581
Only null left
Add to Cart
Edge
Add to Cart

Description

Degrade single-stranded DNA in a 3'→5' direction with Exonuclease I (ExoI), leaving 5'-terminal dinucleotides intact.

Exonuclease I (ExoI) degrades single-stranded DNA in a 3'≥5' direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaves 5'-terminal dinucleotides intact.

It does not cleave DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups.

Highlights

  • Active in PCR buffers

Applications

  • Primer removal from PCR mixtures:
    • prior to PCR product sequencing
      • li>for one-tube "megaprimer" PCR mutagenesis
  • Removal of single-stranded DNA containing a 3'-hydroxyl terminus from nucleic acid mixtures
  • Assay for the presence of single-stranded DNA with a 3'-hydroxyl terminus

Note

The enzyme is not suitable for removing 3'-overhangs of dsDNA.

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

TRUSTED_SUSTAINABILITY

Specifications

Concentration 20 U/μL
Enzyme Exonuclease
Quantity 4,000 units
Product Type Exonuclease I

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.