Thermo Scientific™ Exonuclease III (200 U/μL)

Description

Digest double-stranded DNA or DNA-RNA hybrids with Exonuclease III is a 3'–>5' exonuclease.

Exonuclease III (ExoIII) exhibits four catalytic activities (see Reference1). The 3'≥5' exodeoxyribonuclease activity of ExoIII is specific for double-stranded DNA. ExoIII degrades dsDNA from blunt ends, 5'-overhangs or nicks, releases 5'-mononucleotides from the 3'-ends of DNA strands and produces stretches of single-stranded DNA. It is not active on 3'-overhang ends of DNA that are at least four-bases long and do not carry a 3'-terminal C-residue (see Reference 2) on single-stranded DNA, or on phosphorothioate-linked nucleotides.

ExoIII 3'-phosphatase activity removes the 3'-terminal phosphate, generating a 3'-OH group. ExoIII Rnase H activity exonucleolytically degrades the RNA strand in RNA-DNA hybrids. ExpOOO apurinic/apyrimidinic-endonuclease activity cleaves phosphodiester bonds at apurinic or apyrimidinic sites to produce 5'-termini that are base-free deoxyribose 5'-phosphate residues.

Highlights

• Active in restriction enzyme buffers

Specifications

• Concentration: 200 U/μL
• Enzyme: Exonuclease
• Compatible Buffer: Enzyme Buffer
• Quantity: 4,000 units
• Product Type: Exonuclease III

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.