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Thermo Scientific™ Exonuclease III (200 U/μL)

Catalog No. FEREN0191
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4,000 units
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FEREN0191 4,000 units
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Catalog No. FEREN0191 Supplier Thermo Scientific™ Supplier No. EN0191
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Description

Digest double-stranded DNA or DNA-RNA hybrids with Exonuclease III is a 3'–>5' exonuclease.

Exonuclease III (ExoIII) exhibits four catalytic activities (see Reference1). The 3'≥5' exodeoxyribonuclease activity of ExoIII is specific for double-stranded DNA. ExoIII degrades dsDNA from blunt ends, 5'-overhangs or nicks, releases 5'-mononucleotides from the 3'-ends of DNA strands and produces stretches of single-stranded DNA. It is not active on 3'-overhang ends of DNA that are at least four-bases long and do not carry a 3'-terminal C-residue (see Reference 2) on single-stranded DNA, or on phosphorothioate-linked nucleotides.

ExoIII 3'-phosphatase activity removes the 3'-terminal phosphate, generating a 3'-OH group. ExoIII Rnase H activity exonucleolytically degrades the RNA strand in RNA-DNA hybrids. ExpOOO apurinic/apyrimidinic-endonuclease activity cleaves phosphodiester bonds at apurinic or apyrimidinic sites to produce 5'-termini that are base-free deoxyribose 5'-phosphate residues.

Highlights

  • Active in restriction enzyme buffers
TRUSTED_SUSTAINABILITY

Specifications

Concentration 200 U/μL
Enzyme Exonuclease
Compatible Buffer Enzyme Buffer
Quantity 4,000 units
Product Type Exonuclease III

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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