Gibco™ Expi293™ Met (–) Expression Medium

Catalog No.	Quantity
A4096701	1 L

Catalog No. A4096701 Supplier Gibco™ Supplier No. A4096701

Description

Expi293 Met (–) Expression Medium is a cGMP-manufactured, chemically defined, serum-free, protein-free, animal origin-free, methionine-free medium for growth and transfection of Expi293F cells.

• The medium is part of the Expi293 platform for rapid, high-yield transient protein expression from mammalian HEK293 cells
• Designed for use in combination with the ExpiFectamine 293 Met (–) Transfection Kit for applications where labelling of methionine residues in proteins is desired
• Note: the medium will not support cell growth or expression unless supplemented with methionine

Features:

• Supports growth of suspension 293 cultures to densities of over 1.5 x 10⁷ cells/mL
• Equivalent performance to Expi293 Expression Medium
• Does not contain methionine
• Does not contain phenol red
• Formulated with GlutaMAX I Supplement
• Supplementation with methionine required

Specifications

• Product Type: 293 Expression Medium
• Content And Storage: 1 L Expi293 Met (–) Expression Medium, store at 2–8°C (protect from light)
  
  
• Classification: AOF, Chemically Defined, Methionine-Free, Protein-Free, Serum Free
• Shipping Condition: Room Temperature
• Product Line: Expi293
• Quantity: 1 L
• Form: Liquid

Frequently Asked Questions (FAQs)

I am performing L-selenomethionine labeling and getting very low protein yields. What could be going wrong?

L-selenomethionine is a known cell toxin. Therefore, it is important to grow cells in the present of this amino acid for the shortest amount of time required to achieve maximal labeling. We recommend adding L-selenomethionine to the culture just prior to transfection (in Expi293 Met (-) Expression Medium). You may also starve cells for 6 hr by culturing cells in Expi293 Met (-) Expression Medium (without supplementation) and then proceed with L-selenomethionine add-back and transfection.

I'm culturing my Expi293F cells in Expi293 Met (-) Expression Medium but they are not growing. What could be going wrong?

Expi293 Met (-) Expression Medium does not contain methionine, which is an essential nutrient for cell culture. Therefore, methionine supplementation is required. For methionine labeling, cells should be grown in Expi293 Met (-) Expression Medium and starved (i.e., grown in the absence of methionine supplementation) for 6 hr prior to addition of 13C-methyl-L-methionine, or similar methionine isotope.

How do I use Expi293 Met (-) Expression Medium for methionine protein labeling?

Expi293 Met (-) Expression Medium is not intended to support long-term cell culture as the media is deficient of methionine, an essential amino acid for cell survival. When you are ready to start your labeling experiment, transfer Expi293F Cells growing in Expi293 Expression Medium to Expi293 Met (-) Expression Medium by centrifuging the cell suspension (200 x g for 5 min) and resuspending the cell pellet in Expi293 Met (-) Expression Medium. Cells may be starved for 6 hr prior to supplementation with L-selenomethionine, isotopic-labeled methionine, or other derivatives.

What is the difference between Expi293 Expression Medium and Expi293 Met (-) Expression Medium?

The formulation of Expi293 Met (-) Expression Medium is identical to that of Expi293 Expression Medium except for the absence of methionine. Expi293 Met (-) Expression Medium allows for the addition of isotopic-labeled methionine or derivatives to promote labeling of proteins for structural analysis.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.