MilliporeSigma™ EZ-ChIP™

Description

Includes

• DNA Purification Spin Columns and Collection Tubes
• ChIP Blocked Protein G Agarose
• Anti-RNA Polymerase II
• Control PCR Primers
• Normal Mouse IgG
• Bind, Wash and Elution Reagents
• Protease Inhibitor Cocktail II
• RNase A
• Proteinase K
• All required buffers
• ChIP Dilution Buffer
• Low Salt Immune Complex Wash Buffer
• High Salt Immune Complex Wash Buffer
• LiCl Immune Complex Wash Buffer
• TE Buffer
• 0.5M EDTA
• 5M NaCl
• SDS Lysis Buffer
• 1M Tris-HCl, pH 6.5
• 10X PBS
• 10X Glycine
• 1M NaHCO3
• Control Primers
• 20% SDS
• Spin Filters
• Collection Tubes
• Bind Reagent A
• Wash Reagent B
• Elution Reagent C

Any chromatin-associated or DNA binding protein can be analyzed with this technique, provided a good antibody to the protein exists

• Easier: Spin columns make DNA purification easier and more reliable - no more messy phenol-chloroform extractions
• Quicker: All reagents to process your samples are included - you don't have to spend valuable time making them
• Greater Reproducibility: Positive and negative control antibodies and PCR primers are included to help validate your results and to troubleshoot your experiments

Specifications

• Content And Storage: Store components at the temperatures indicated on the labels
• For Use With (Application): 22 individual chromatin immunoprecipitation (ChIP) reactions using inexpensive protein G agarose beads
• Quantity: 22 Assays
• Stability: Stable for 1 year from date of shipment when stored as directed