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  6. Thermo Scientific™ EZ-Link™ Maleimide-PEG4-DBCO

Thermo Scientific™ EZ-Link™ Maleimide-PEG4-DBCO

Catalog No. FERC20044
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25 mg
10 x 1 mg
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FERC20044 10 x 1 mg
FERC20041 25 mg
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Catalog No. FERC20044 Supplier Thermo Scientific™ Supplier No. C20044
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Description

A sulfhydryl reactive heterobifunctional crosslinker which contains a dibenzylcyclooctyne (DBCO) group for copper-free click chemistry applications. The thiol/sulfhdryl reactive maleimide can be used to label a variety of thiol containing moieties such as free cystines in proteins, peptides, or other (bio)molecules.

  • A sulfhydryl reactive heterobifunctional crosslinker which contains a dibenzylcyclooctyne (DBCO) group for copper-free click chemistry applications
  • The thiol/sulfhdryl reactive maleimide can be used to label a variety of thiol containing moieties such as free cystines in proteins, peptides, or other (bio)molecules
  • Once a protein or (bio)molecule is DBCO labeled, it can undergo a copper-free azide-alkyne cycloaddition reaction to form a stable triazole linkage with an azide labeled coupling partner
  • Can be used to create diverse conjugates with the biorthogonal coupling between an alkyne and azide (supplied separately)
  • The coupling or crosslinking procedure is typically done in two steps:
    • First, a protein or thiol containing molecule is labeled with a DBCO group and excess crosslinker is removed
    • Second, an azide containing moiety (prepared independently) is introduced into the same system as the DBCO moiety
    • Labeling occurs under biological conditions without the introduction of any additional reagents such as a copper catalyst
  • Reagent readily dissolves in water-miscible solvents for dilution into aqueous reaction mixtures
TRUSTED_SUSTAINABILITY

Specifications

Chemical Reactivity Azide-Sulfhydryl
Cleavable No
Description Maleimide-PEG4-DBCO
Molecular Weight (g/mol) 647.74
PEGylated Yes
Spacer Arm Length 29.7 Å
Content And Storage Upon receipt store desiccated at -20°C.
Cell Permeability Not determined
Shipping Condition Wet Ice
Product Line EZ-Link No-Weigh
Labeling Method Chemical Labeling
Crosslinker Type Heterobifunctional
Reactive Moiety Maleimide, DBCO
Spacer Medium (10 to 30 Å)
Form Powder
Quantity 10 x 1 mg
Solubility DMF, DMSO
Format Standard, Single-use
Water Soluble No
Product Type Crosslinker
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