MilliporeSigma™ EZ-Magna ChIP™ HiSens Chromatin Immunoprecipitation Kit

Description

Includes

• 10X Glycine
• Nuclei Isolation Buffer
• 10X PBS
• SCW Buffer (Sonication/ChIP/Wash)
• Magna ChIP Protein A/G Magnetic Beads
• Low Stringency IP Wash Buffer
• ChIP Elution Buffer
• Protease Inhibitor Cocktail
• Proteinase K Solution
• Anti-Trimethyl-Histone H3 (Lys4)
• Normal Rabbit IgG
• Control PCR primers

Requires

Reagents

• Cells, stimulated or treated as desired
• Antibody of interest for chromatin immunoprecipitation
• 37% Formaldehyde
• qPCR reagents, such as SYBR™ Green Master Mix
• 2% Agarose gel
• 100 bp DNA Ladder
• 6X gel Loading buffer
• DNase- and RNase-free sterile H2O

Specialized blend of protein A/G blend of magnetic beads is specifically produced for chromatin immunoprecipitation and enables the use of a broader range of antibodies, eliminating the need to purchase different kits for different antibody isotypes.

• Robust ChIP from as few as 10,000 to as many as 1,000,000 cells
• Protocols and reagents for generation of chromatin from range of input sample type
• Superior performance with variety of isotypes of either polyclonal or monoclonal antibodies
• Specialized buffer and bead formulations; lower backgrounds and higher fold enrichment
• Single buffer system for sonication, chromatin IP, and wash
• Perform analysis of enrichment without additional purification after cross link reversal
• Compatible with all commonly used downstream analysis applications– qPCR, next generation sequencing, microarray
• Includes positive and negative control antibodies and assay against known positive locus

Specifications

• Content And Storage: Store components at the temperatures indicated on the labels
• For Use With (Application): To enable ChIP from low input amounts of chromatin obtained from either cells or tissues using magnetic A/G beads
• Quantity: 24 Assays
• Stability: Kit components are stable for 1 year from date of shipment when stored as directed