MilliporeSigma™ EZ-Magna Nuclear RIP™ (Cross-Linked) Nuclear RNA-Binding Protein Immunoprecipitation Kit

Catalog No. 17-105-21

Supplier: MilliporeSigma™ 1710521

Description

Includes

• 10X Glycine
• 10X PBS
• Nuclei Isolation Buffer
• RIP Cross-Linked Lysis Buffer
• Protein A/G Magnetic Beads
• Nuclear RIP Dilution Buffer
• Low Salt Wash Buffer
• High Salt Wash Buffer
• LiCl Wash Buffer
• TE Buffer
• RIP Elution Buffer
• 10% SDS
• 0.5 M EDTA
• DNase I (RNase Free)
• DNase I Supplement
• DNase I Reaction Buffer
• Protease Inhibitor Cocktail III, Animal Free
• RNAse Inhibitor
• Proteinase K

Requires

• Antibody of interest for RNA-binding protein immunoprecipitation (RIP)
• Negative Control Antibody
• Precipitation Carrier (e.g. Pellet Paint™ Co-Precipitant or linear Acrylamide (5mg/mL) if samples are intended for Next Gen Sequence library preparation
• RNase A (10mg/mL)
• Reagents for qRT-PCR Analysis and/or Reagents for RNA-Seq Library Construction

Specially designed to allow the discovery and analysis of a variety of chromatin associated RNAs such as long non-coding RNAs, enhancer RNAs and miRNAs

• Generates cross-linked chromatin to allow analysis of a variety of chromatin-associated RNAs
• Flexible, scalable input requirements: Recover RNA from millions of cells or as few as 5,000 cells
• Magnetic protein A/G bead blend and optimized buffer system results in low backgrounds and high signal-to-noise ratios
• Suitable for analysis by RT-qPCR or RIP-seq
• Complete set of reagents and detailed protocol to enable first-time success

Specifications

• Content And Storage: Store components at the temperatures indicated on the labels
• For Use With (Application): RNA Binding Protein Immunoprecipitation (RIP), Protein Interaction Assays
• Quantity: 24 Assays
• Stability: Kit components are stable for 6 months from date of shipment when stored as directed