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MilliporeSigma™ EZ-Magna Nuclear RIP™ (Native) Nuclear RNA-Binding Protein Immunoprecipitation Kit
Specially designed to allow the discovery and analysis of a variety of chromatin associated RNAs such as long non-coding RNAs, enhancer RNAs and miRNAs
Supplier: MilliporeSigma™ 1710523
Includes:
- Nuclei Isolation Buffer
- RIP Lysis Buffer
- Protein A/G Magnetic Beads
- RIP Lysis Buffer
- 0.5M EDTA
- DNase I (RNase Free) 2U/μL
- DNase I Reaction Buffer
- Protease Inhibitor Cocktail III, Animal Free
- RNAse Inhibitor
Requires :
- Antibody of interest for RNA-binding protein immunoprecipitation (RIP)
- Negative Control Antibody
- Precipitation Carrier (e.g. Pellet Paint™ Co-Precipitant or linear Acrylamide (5mg/mL) if samples are intended for Next Gen Sequence library preparation
- Reagents for qRT-PCR Analysis and/or Reagents for RNA-Seq Library Construction (optional)
Description
- Generates native chromatin to allow analysis of a variety of chromatin-associated RNAs
- Flexible, scalable input requirements: Recover RNA from millions of cells or as few as 100,000 cells (native)
- Magnetic protein A/G bead blend and optimized buffer system results in low backgrounds and high signal-to-noise ratios
- Suitable for analysis by RT-qPCR or RIP-seq
- Complete set of reagents and detailed protocol to enable first-time success
- Includes positive and negative control antibodies and qPCR primers
Specifications
| Store components at the temperatures indicated on the labels | |
| RNA Binding Protein Immunoprecipitation (RIP), Protein Interaction Assays | |
| 24 Assays | |
| Kit components are stable for 6 months from date of shipment when stored as directed |
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