Sigma Aldrich Fine Chemicals Biosciences EZMTT High Sensitivity Cel

Supplier:  Sigma Aldrich Fine Chemicals Biosciences CBA411

NAD/NADH and NADP/NADPH are important biological cofactors foroxidoreductase enzymes that arefundamental for various biological processes such as energy metabolism mitochondrial function oxidative stress immunological functions and cell death. Hence the oxidoreductase enzymes are used as indicators of metabolic activity of cells.Traditional tetrazolium salts such as MTT react with NAD(P)H-dependent cellular oxidoreductase enzymes to produce an insoluble formazan which requires solubilization and have lower sensitivity. Moreover they cannot reliably detect cell viability in low density cultures such as primary cell cultures. EZMTT viability assay SOLUTIONon is based on a novel monosulfonated tetrazolium salt 2-(3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazol-3-ium-5-yl) benzenesulfonate sodium salt (EZMTT). The EZMTT reagents provided high quality and reproducible detection for oxidoreductase enzymes indicators of cellular metabolic activity. Upon reacting with meta

Catalog No. 50-290-6110