Invitrogen™ FAP Human ELISA Kit

Sandwich ELISA Kit

Manufacturer:  Invitrogen™ EHFAP

Catalog No. EHFAP

Description

The Human FAP ELISA quantitates Hu FAP in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu FAP. Principle of the method The Hu FAP solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis.

Specifications

• For Use With (Equipment): Colorimetric Microplate Reader
• Specificity: This ELISA antibody pair detects human FAP. Other species not determined yet.
• Assay: Solid-phase sandwich ELISA
• Protein Family: angiogenesis; cell adhesion; dipeptidyl-peptidase activity; endopeptidase activity; endothelial cell migration; melanocyte apoptotic process; melanocyte proliferation; metalloendopeptidase activity; mitotic cell cycle arrest; negative regulation of cell proliferation involved in contact inhibition; negative regulation of extracellular matrix disassembly; negative regulation of extracellular matrix organization; peptidase activity; positive regulation of cell cycle arrest; positive regulation of execution phase of apoptosis; proteolysis; proteolysis involved in cellular protein catabolic process; regulation of collagen catabolic process; regulation of fibrinolysis; serine-type endopeptidase activity; serine-type peptidase activity
• Accession Number: Q12884
• Calibration Range: 12pg/mL to 4000pg/mL
• Gene ID (Entrez): 2191
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Total Assay Time: 4 hr. 45 min.
• Research Discipline: Angiogenesis
• Interassay CV: <12%
• Quantity: 96 Tests
• Sample Type: Plasma, Serum, Supernatant
• Gene Symbol: FAP

• Product Type: ELISA Kit
• Sufficient For: 96 assays
• Conjugate: Biotin
• Label or Dye: HRP
• Assay Range: 16.38-4000 pg/mL
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Sample Volume: Plasma, 1 μL; Serum, 1 μL; Supernatant, 50 μL
• Test Time: 1 hr. 20 min.
• Target: Human
• Synonym: DPPIVA, SIMP, FAP
• Intraassay CV: <10%
• Target Species: Human
• Assay Sensitivity: 12 pg/mL