Applied Biosystems™ Fast MicroSEQ™ D2 Fungal rDNA PCR Kit

Description

This kit is the PCR component of the FAST MicroSEQ Fungal Identification System, with enough reagents for 50 PCR amplifications of fungal DNA unknowns.

This kit is the PCR component of the FAST MicroSEQ™ Fungal Identification System, with enough reagents for 50 PCR amplifications of fungal DNA unknowns. The sequencing component of the kit is required for species identification. Together, the two kits provide an easy-to-use DNA sequence-based method to identify most fungi.

• Sequence-based measurement ensures you get reproducible results in less than 24 hours.
• Growth requirement-independent measurement enables you to identify fungi using objective criteria.
• Universal domain-level primers eliminate up-front optimization.
• MicroSEQ™ ID Analysis Software ensures accurate species identification.
• Customizable libraries give you the flexibility to create new libraries and search validated libraries that contain the most frequently encountered organisms.
• Requires minimal training; same work flow as the MicroSEQ™ Full Gene 16S rDNA Bacterial Identification Kit.

Specific and Easy to Use

Based on phylogenetic analysis of the D2 region of large-subunit ribosomal RNA gene sequences, this easy-to-use kit enables you to identify even the most difficult fungi, yeast, and oomycete plant pathogens. To ensure accurate and specific results, the kit's software compares your samples to a database of D2 sequences from over 1000 validated type species.

The MicroSEQ™ ID System: A Complete, Integrated Solution

Streamlining every step in microbial identification, Applied Biosystems™ MicroSEQ™ System combines the advantages of MicroSEQ™ ID Software with easy PrepMan Ultra sample preparation reagents and protocols, MicroSEQ™ bacterial and fungal application kits, and industry-leading thermal cyclers and sequencing systems. To ensure optimal performance and system-wide integration, we develop and test all components of the system together.

Specifications

• Content And Storage: The kit contains enough reagent for 50 identifications:
  • PCR Master Mix (store at 2°C to 8°C in PCR clean room)
  • Positive PCR Control DNA (2°C to 8°C in PCR clean room)
  • Negative PCR Control (2°C to 8°C in PCR clean room)
• Detection Method: Primer-probe
• GC-Rich PCR Performance: Low
• Reaction Speed: Fast
• Technique: SBT (Sequence-Based Typing)
• For Use With (Equipment): GeneAmp 9700, Veriti Thermal Cycler, 9800 Fast Thermal Cycler
• Libraries: Fungal Library (> 1100 species), Customizable
• Product Line: MicroSEQ™
• Product Type: rDNA PCR Kit
• Quantity: 1 Kit
• Shipping Condition: Dry Ice
• Software: MicroSEQ™ ID Analysis Software
• Target: D2 region of rRNA gene
• Target Organism Class: Fungi
• Target Specificity: Target-Specific
• For Use With (Application): Standard PCR
• Sample Type: DNA (Genomic)

For Research Use Only. Not for use in diagnostic procedures.