Invitrogen™ Fc Receptor Binding Inhibitor Polyclonal Antibody, Functional Grade, eBioscience™

Polyclonal Antibody

Supplier:  Invitrogen™ 16916173

Catalog No. 50-112-9027

Description

Description: The human FcgammaR-binding inhibitor can be used to inhibit the non-specific Fc-gamma receptor (FcgammaR)-mediated binding of mouse monoclonal antibodies used for flow cytometric analysis of human tissue. Four different classes of Fcgamma receptors have been identified: FcgammaRI (CD64), FcgammaRII (CD32), and FcgammaRIII (CD16), and they are expressed at varying levels in multiple cell lineages including high expression in myeloid, granulocyte and B cells. The biological function of the FcgammaR, including initiation of endocytosis, phagocytosis and antigen presentation, is elicited upon binding of host-immunoglobulin. The extent to which mouse monoclonal antibodies will bind to human FcgammaR varies depending on the isotype of the monoclonal antibody. Furthermore, different monoclonal antibodies of the same isotype will display different binding to human FcgammaRs. Applications Reported: FG Purified Human Fc Receptor Binding Inhibitor has been reported for use in flow cytometric analysis. To inhibit the non-specific binding of mouse monoclonal antibodies add the human Fc Receptor Binding Inhibitor to samples and incubate on ice for 20 minutes. Without washing proceed to stain with primary antibody. Applications Tested: FG Purified Human FcgR-Binding Inhibitor has been pre-titrated and tested by inhibiting binding of fluorochrome-conjugated isotype controls to U937 cells. This can be used at 20 μL per test. A test is defined as the amount (μg) of antibo...

Four different classes of Fcy receptors have been identified: FcyRI (CD64), FcyRII (CD32), and FcyRIII (CD16), and they are expressed at varying levels in multiple cell lineages including high expression in myeloid, granulocyte and B cells. The biological function of the FcyR, including initiation of endocytosis, phagocytosis and antigen presentation, is elicited upon binding of host-immunoglobulin. The extent to which mouse monoclonal antibodies will bind to human FcyR varies depending on the isotype of the monoclonal antibody. Furthermore, different monoclonal antibodies of the same isotype will display different binding to human FcyRs.

Specifications

• Antigen: Fc Receptor Binding Inhibitor
• Classification: Polyclonal
• Conjugate: Functional Grade
• Gene Alias: Fc blocking; FcR BI; FcR Inhibitor
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: Human
• Product Type: Antibody
• Green Features: Responsibly packaged

• Applications: Control, Flow Cytometry, Functional Assay
• Concentration: 20 μL/Test
• Formulation: PBS with no preservative; pH 7.2
• Host Species: Not Available
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Content And Storage: 4°C
• Form: Liquid