Learn More
Thermo Scientific™ Aminoallyl-dUTP Solution (50 mM)
Description
Thermo Scientific Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as 10 mM and 50 mM aqueous solutions titrated to pH 7.0 with NaOH. Aminoallyl-dUTP can be enzymatically incorporated into DNA and the resulting amine-containing DNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin or hapten.
Applications
• Enzymatic indirect non-radioactive labeling of DNA during cDNA synthesis
• PCR
• Nick-translation
• Random-primed labeling
• Primer extension
Aminoallyl-dUTP can be enzymatically incorporated into DNA with:
• Reverse transcriptases
• Taq DNA polymerase (Cat. No. EP0072)
• phi29 DNA Polymerase (EP0091)
• Klenow Fragment (EP0051)
• Klenow Fragment, exo- (EP0421)
• DNA Polymerase I (EP0041)
Specifications
Specifications
| Concentration | 50 mM |
| Content And Storage | Aminoallyl-dUTP Solution, 50 mM (50 μL) Store at –20°C. |
| Form | Liquid |
| Polymerase | DNA Polymerase |
| Labeling Method | Direct Labeling |
| Label or Dye | Aminoallyl |
| Quantity | 50 μL |
| Shipping Condition | Dry Ice |
| For Use With (Application) | Standard PCR, Gene Expression, Non-Radioactive Labeling |
For Research Use Only. Not for use in diagnostic procedures.
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.