Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Thermo Scientific™ Eco81I (Bsu36I) (10 U/μL)

Catalog No. FERER0371
Click to view available options
Quantity:
2,500 units
500 units

The Eco81I (Bsu36I) restriction enzyme recognizes CC^TNAGG sites and cuts best at 37°C in Tango buffer (Isoschizomers: AxyI, Bse21I, Bsu36I).

Lambda DNA digested with Eco81I (Bsu36I), 0.7% agarose, 2 cleavage sites

conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'...C C▵T N A G G...3'
  • 3'...G G A N T▵C C...5' Conditions for 100% Activity:
    • 1X Buffer Tango: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
    • Incubate at 37°C
    Storage Buffer:
    • Eco81I is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol
    Ligation and Recleavage:
    • After 10-fold overdigestion with Eco81I, more than 80% of the M13mp18 DNA fragments can be ligated in a reaction mixture containing 20-40U of T4 DNA Ligase/1μg of fragments and 10% PEG
    • More than 95% of these can be recut
    Methylation Effects:
    • Dam: never overlaps-no effect
    • Dcm: never overlaps-no effect
    • CpG: never overlaps-no effect
    • EcoKI: never overlaps-no effect
    • EcoBI: may overlap-effect not determined
    Digestion of Agarose-embedded DNA:
    • Minimum 5U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours
    Compatible Ends:
    • BbvCI, Bpu10I, Bpu1102I, HpyF3I
  • TRUSTED_SUSTAINABILITY

    Specifications

    Concentration 10 U/μL
    Methylation Sensitivity Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
    Enzyme Eco81I (Bsu36I)
    Compatible Buffer 10X Buffer Tango
    Sensitive to Heat Inactivation Yes
    Optimal Reaction Temperature 37°C
    Type IIS RE No
    Quantity 500 units
    Product Type Restriction Enzyme
    Research Category Traditional Cloning

    For Research Use Only. Not for use in diagnostic procedures.

    Product Content Correction

    Your input is important to us. Please complete this form to provide feedback related to the content on this product.

    Product Title

    By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

    Your feedback has been submitted: Thank you for helping us improve our website.