Thermo Scientific™ Eco81I (Bsu36I) (10 U/μL)

Description

The Eco81I (Bsu36I) restriction enzyme recognizes CC^TNAGG sites and cuts best at 37°C in Tango buffer (Isoschizomers: AxyI, Bse21I, Bsu36I).

Lambda DNA digested with Eco81I (Bsu36I), 0.7% agarose, 2 cleavage sites

conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'...C C▵T N A G G...3'

3'...G G A N T▵C C...5' Conditions for 100% Activity:

• 1X Buffer Tango: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
• Incubate at 37°C

Storage Buffer:

• Eco81I is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

• After 10-fold overdigestion with Eco81I, more than 80% of the M13mp18 DNA fragments can be ligated in a reaction mixture containing 20-40U of T4 DNA Ligase/1μg of fragments and 10% PEG
• More than 95% of these can be recut

Methylation Effects:

• Dam: never overlaps-no effect
• Dcm: never overlaps-no effect
• CpG: never overlaps-no effect
• EcoKI: never overlaps-no effect
• EcoBI: may overlap-effect not determined

Digestion of Agarose-embedded DNA:

• Minimum 5U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours

Compatible Ends:

• BbvCI, Bpu10I, Bpu1102I, HpyF3I

Specifications

• Concentration: 10 U/μL
• Methylation Sensitivity: Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
• Enzyme: Eco81I (Bsu36I)
• Compatible Buffer: 10X Buffer Tango
• Sensitive to Heat Inactivation: Yes
• Optimal Reaction Temperature: 37°C
• Type IIS RE: No
• Quantity: 500 units
• Product Type: Restriction Enzyme
• Research Category: Traditional Cloning

For Research Use Only. Not for use in diagnostic procedures.