Thermo Scientific™ FastDigest KflI

Description

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

The Gibco CTS Xenon MultiShot Electroporation Cartridge is for use with the Xenon Electroporation System for cell therapy research and development. The single-use cartridge has an electroporation chamber made of Tritan™ MX711 copolyester with gold-plated electrodes and is sterilized by gamma irradiation. It can process 1 x 10⁸ to 2.5 x 10⁹ T cells in a closed system with the flexibility of sterile welding to either PVC or CFlex™ tubing.

Promising electroporation conditions that have been optimized in the Neon Transfection System can be scaled to the Xenon Electroporation System without sacrificing performance.

View an interactive 3D tour of the CTS Xenon instrument

Tritan is a trademark of Tritan Technology Inc. C-Flex is a trademark of SaintGobain Performance Plastics Corporation.

The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment (Cat. No. EP0051), T4 DNA Ligase (Cat. No. EL0014), alkaline phosphatases and T4 DNA Polymerase (Cat. No. EP0061) all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.

Features
• 100% activity of all FastDigest enzymes in the universal buffer
• 100% buffer compatibility with downstream applications
• Complete digestion in 5–15 minutes
• Direct loading on gels
• No star activity
• 176 FastDigest enzymes available

Applications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern Blot
• Restriction fragment length polymorphism (RFLP)
• SNP analysis

Note: The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.

For methylation sensitivity, refer to product specifications.

Specifications

• Content And Storage: • FastDigest KflI (20 μL)
  • 10X FastDigest Buffer (1.0 mL)
  • 10X FastDigest Green Buffer (1.0 mL)
  
  Store at –20°C.
  
  
• Methylation Sensitivity: CpG Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dam Methylation-Sensitive
• Enzyme: KflI
• Compatible Buffer: 10X FastDigest Buffer/FastDigest Green Buffer
• Sensitive to Heat Inactivation: No
• Optimal Reaction Temperature: 37°C
• Type IIS RE: No
• Quantity: 20 μL (20 Reactions)
• Product Type: Restriction Enzyme
• Research Category: Traditional Cloning
• Product Line: FastDigest™
• Overhang: 5' protruding ends

For Research Use Only. Not for use in diagnostic procedures.