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Thermo Scientific™ Fluorescein-12-dUTP Solution (1 mM)
Description
Thermo Scientific Fluorescein-12-dUTP (fluorescein-5(6)-carboxaminocaproyl-[5-{3-aminoallyl}-2'-deoxyuridine-5-triphosphate]) is supplied as 1 mM aqueous solution.
Fluorescein-12-dUTP can be enzymatically incorporated into DNA with:
• Reverse transcriptases
• Taq DNA polymerase (Cat. No. EP0072)
• phi29 DNA Polymerase (EP0091)
• Klenow Fragment, exo- (EP0421)
• Klenow Fragment (EP0051)
• DNA Polymerase I (EP0041)
Applications
• Enzymatic non-radioactive labeling of DNA during cDNA synthesis
• PCR
• Nick-translation
• Random primed labeling
• Primer extension
Specifications
Specifications
| Concentration | 1 mM |
| Content And Storage | Fluorescein-12-dUTP Solution, 1 mM (25 μL) Store at –20°C. |
| Form | Liquid |
| Polymerase | DNA Polymerase |
| Labeling Method | Direct Labeling |
| Label or Dye | Fluorescein (FITC) |
| Quantity | 25 μL |
| Shipping Condition | Dry Ice |
| For Use With (Application) | cDNA Synthesis, Non-Radioactive Labeling |
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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