Thermo Scientific™ KpnI (10 U/μL)

Description

The KpnI restriction enzyme recognizes GGTAC^C sites and cuts best at 37°C in its own unique buffer (Isoschizomers: Asp718I).

Lambda DNA digested with KpnI, 0.7% agarose, 2 cleavage sites

conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'..G G T A C▵C...3'

3'..C▵C A T G G...5'

Conditions for 100% Activity:

• 1X Buffer KpnI: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl₂, 0.02% Triton X-100 and 0.1mg/mL BSA
• Incubate at 37°C

Storage Buffer:

• KpnI is supplied in: 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

• Dam: never overlaps —no effect
• Dcm: may overlap —no effect
• CpG: may overlap —no effect
• EcoKI: never overlaps —no effect
• EcoBI: never overlaps —no effect

Digestion of Agarose-embedded DNA:

• Minimum 5U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours

Note:

High glycerol (>5%) concentrations, pH>8.0 or a large excess of enzyme may result in star activity.

Specifications

• Concentration: 10 U/μL
• Methylation Sensitivity: Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
• Enzyme: Kpn I
• Compatible Buffer: Unique Buffer (10X Buffer KpnI)
• Sensitive to Heat Inactivation: Yes
• Optimal Reaction Temperature: 37°C
• Type IIS RE: No
• Quantity: 4,000 units
• Product Type: Restriction Enzyme
• Research Category: Traditional Cloning

For Research Use Only. Not for use in diagnostic procedures.