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Thermo Scientific™ M13/pUC Reverse Sequencing Primer (-26), 17-mer
Description
Thermo Scientific M13/pUC Reverse Sequencing Primer (-26), 17-mer is a synthetic single-stranded 17-mere oligonucleotide with free 5'- and 3'-hydroxyl ends. This M13/pUC primer anneals to the region in the 5'-terminus of the lacZ gene. All primers are supplied as 10 μM aqueous solutions.
Applications
• Sequencing of DNA fragments inserted into the MCS within the lacZ gene of various cloning vectors, such as pUC19 (Cat. No. SM0221), pTZ19R, pTZ57R, M13mp18, and pBluescript II
• Colony screening by PCR
Primer Sequence: 5'-d(CAGGAAACAGCTATGAC)-3'

Specifications
Specifications
Product Type | Sequencing Primer |
Content And Storage | M13/pUC Reverse Sequencing Primer (-26), 17-mer, 10 μM (60 μL) Store at –20°C. |
Shipping Condition | Dry Ice |
Concentration | 10 μM |
Primer | M13 |
Quantity | 10 μM, 60 μL |
Vector | pUC19, pTZ19R, pTZ57R, M13mp18, pBluescript II |
For Use With (Application) | Sequencing |
Form | Liquid |
For Research Use Only. Not for use in diagnostic procedures.
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