Thermo Scientific™ RevertAid H Minus First Strand cDNA Synthesis Kit

Description

Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.

Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit includes RevertAid H Minus Reverse Transcriptase, which has a point mutation that completely eliminates RNase H activity. Therefore, it does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA.

Features of the RevertAid H Minus First Strand cDNA Synthesis Kit include:
• High yields of full-length first-strand cDNA up to 13 kb
• Increased reaction temperatures of 42–55°C
• Supplied with the recombinant RiboLock RNase Inhibitor
• Complete kit includes oligo(dT)₁₈ and random hexamer primers

Applications
• First strand cDNA synthesis for RT-PCR and RT-qPCR
• Construction of full length cDNA libraries
• Antisense RNA synthesis

Additional Features
The recombinant RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with the reverse transcription reaction, as it maintains activity at temperatures up to 55°C. The kit is supplied with both oligo(dT)₁₈ and random hexamer primers. The oligo(dT)₁₈ primer anneals selectively to the poly(A) tail of mRNA. Random hexamer primers do not require the presence of poly(A), therefore, they can be used for transcription of the 5’-end regions of mRNA or for cDNA synthesis using RNA without the poly(A) tail, e.g., microRNAs. Gene-specific primers may also be used with the kit. The first strand cDNA can be directly used as a template in PCR, real-time PCR, or in second strand cDNA synthesis.

Specifications

• Content And Storage:

  • RevertAid H Minus Reverse Transcriptase
  • RiboLock RNase Inhibitor
  • 5X Reaction Buffer
  • dNTP Mix
  • Oligo(dT)₁₈ Primer
  • Random Hexamer Primer
  • Control RNA
  • Control Primers
  • Nuclease-free water
  
  Store at –20°C.
  
  

• Format: Kit
• Reaction Speed: 60 min.
• Technique: Reverse Transcription
• Optimal Reaction Temperature: 42°C to 45°C
• Quantity: 100 reactions
• Reverse Transcriptase: RevertAid H Minus
• Ribonuclease H Activity: Reduced
• Shipping Condition: Dry Ice
• For Use With (Application): Real Time PCR (qPCR), RT-PCR
• Final Product Type: First-Strand cDNA
• No. of Reactions: 100 Reactions
• Reaction Format: Separate components
• Reagent Type: Reverse Transcription
• Size (Final Product): Up to 13 kb
• Starting Material: RNA

For Research Use Only. Not for use in diagnostic procedures.