Thermo Scientific™ RevertAid Reverse Transcriptase (200 U/μL)

Description

Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties.

Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids that is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase.

Features of RevertAid Reverse Transcriptase include:
• Efficient synthesis of full-length first strand cDNA up to 13 kb
• Optimum activity at 42°C
• Active up to 50°C
• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, and fluorescein-labeled nucleotides)

Applications
• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Synthesis of cDNA for cloning and expression
• Generation of labeled cDNA probes for microarrays
• DNA labeling
• Analysis of RNA by primer extension

Source
RevertAid Reverse Transcriptase is sourced from E.coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptase.

Specifications

• Concentration: 200 U/μL
• Content And Storage:

  • RevertAid Reverse Transcriptase (200 U/μL)
  • 5X Reaction Buffer
  
  Store at –20°C.
  
  

• Format: Stand-alone Enzyme
• Reaction Speed: 60 min.
• Technique: Reverse Transcription
• Optimal Reaction Temperature: 42°C
• Quantity: 5 x 10,000 Units
• Reverse Transcriptase: RevertAid
• Ribonuclease H Activity: Yes
• Shipping Condition: Dry Ice
• Final Product Type: First-Strand cDNA
• Reaction Format: Separate components
• Reagent Type: Reverse Transcription
• Size (Final Product): Up to 13 kb
• Starting Material: RNA

For Research Use Only. Not for use in diagnostic procedures.