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Thermo Scientific™ RevertAid Reverse Transcriptase (200 U/μL)

Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties.

$125.50 - $517.50

Specifications

Content And Storage

• RevertAid Reverse Transcriptase (200 U/μL)
• 5X Reaction Buffer

Store at –20°C.

Format Stand-alone Enzyme
Reaction Speed 60 min.
Technique Reverse Transcription
Optimal Reaction Temperature 42°C
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Catalog Number Mfr. No. Quantity Price Quantity & Availability  
FEREP0441
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Thermo Scientific™
EP0441
10,000 Units
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FEREP0442
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Thermo Scientific™
EP0442
5 x 10,000 Units
Each for $517.50
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Description

Description

Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids that is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase.

Features of RevertAid Reverse Transcriptase include:
• Efficient synthesis of full-length first strand cDNA up to 13 kb
• Optimum activity at 42°C
• Active up to 50°C
• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, and fluorescein-labeled nucleotides)

Applications
• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Synthesis of cDNA for cloning and expression
• Generation of labeled cDNA probes for microarrays
• DNA labeling
• Analysis of RNA by primer extension

Source
RevertAid Reverse Transcriptase is sourced from E.coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptase.

Specifications

Specifications

• RevertAid Reverse Transcriptase (200 U/μL)
• 5X Reaction Buffer

Store at –20°C.

60 min.
42°C
Yes
200 U/μL
Separate components
Up to 13 kb
Stand-alone Enzyme
Reverse Transcription
RevertAid
Dry Ice
First-Strand cDNA
Reverse Transcription
RNA
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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