Thermo Scientific™ RevertAid H Minus Reverse Transcriptase (200 U/μL)

Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain.

Supplier:  Thermo Scientific™ EP0452

Catalog No. FEREP0452

Description

Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain. RevertAid H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA, and therefore high yields of full-length cDNA from long templates are obtained. RevertAid H Minus Reverse Transcriptase maintains activity over a wide temperature range (42–50°C).

Features of RevertAid H Minus Reverse Transcriptase include:
• High yields of full-length first strand cDNA up to 13 kb
• Optimum activity at 42–45°C
• Active up to 55°C
• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, and fluorescein-labeled nucleotides)

Applications
• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Reverse transcription at elevated temperatures to reduce effects of secondary structure
• Synthesis of cDNA for cloning and expression
• Generation of labeled cDNA probes for microarrays
• DNA labeling
• Analysis of RNA by primer extension

Specifications

• Content And Storage:

  • RevertAid H Minus Reverse Transcriptase (200 U/μL)
  • 5X Reaction Buffer
  
  Store at –20°C.
  
  

• Reaction Speed: 60 min.
• Optimal Reaction Temperature: 42°C to 45°C
• Reverse Transcriptase: RevertAid H Minus
• Shipping Condition: Dry Ice
• Final Product Type: First-Strand cDNA
• Reagent Type: Reverse Transcription
• Starting Material: RNA

• Format: Stand-alone Enzyme
• Technique: Reverse Transcription
• Quantity: 5 x 10,000 Units
• Ribonuclease H Activity: Reduced
• Concentration: 200 U/μL
• Reaction Format: Separate components
• Size (Final Product): Up to 13 kb

For Research Use Only. Not for use in diagnostic procedures.