Thermo Scientific™ S1 Nuclease (100 U/μL)

Description

Degrade single-stranded DNA and RNA with this endonuclease.

S1 Nuclease degrades single-stranded nucleic acids, releasing 5'-phosphoryl mono- or oligonucleotides. It is five times more active on DNA than on RNA . S1 Nuclease also cleaves dsDNA at the single-stranded region caused by a nick, gap, mismatch or loop. S1 Nuclease exhibits 3'-phosphomonoesterase activity.

The enzyme is a glycoprotein with carbohydrate content of 18%.

• Source: Aspergillus oryzae
• Molecular Weight: 29 kDa monomer

Quality Control:

• The absence of contaminating double-stranded DNA specific nuclease activity confirmed by appropriate quality test
• Functionally tested for the generation of unidirectional deletions in DNA fragments (in conjunction with Exonuclease III)

Source:

• Aspergillus oryzae

Molecular Weight:

• 29 kDa monomer

Definition of Activity Unit:

• One unit of the enzyme produces 1μg of acid soluble deoxyribonucleotides in 1 min. at 37°C
• Enzyme activity is assayed in the following mixture: 30 mM sodium acetate (pH 4.5), 50 mM NaCl, 0.1 mM ZnCl₂, 5% (v/v) glycerol, 800μg/ml heat denatured calf thymus DNA

Storage Buffer:

• The enzyme is supplied in:20mM Tris-HCl (pH 7.5), 50mM NaCl, 0.1mM ZnCl₂ and 50% (v/v) glycerol

5X Reaction Buffer:

• 200mM sodium acetate (pH 4.5 at 25°C), 1.5M NaCl and 10 mM ZnSO₄

Inhibition and Inactivation:

• Inhibitors: metal chelators, PP_i, P_i, 5'-ribonucleotides and deoxyribonucleotides
• Inactivated by heating at 70°C for 10 min. in the presence of EDTA

Recommended for:

Removal of single-stranded overhangs of DNA fragments (2); S1 transcript mapping (3, 4); Cleavage of hairpin loops; Creation of unidirectional deletions in DNA fragments in conjunction with Exonuclease III (5)

Note:

S1 Nuclease can introduce breaks into double-stranded DNA, RNA and DNA/RNA hybrids at high enzyme and low salt concentrations (6).

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Specifications

• Concentration: 100 U/μL
• Enzyme: Nuclease
• Compatible Buffer: Storage Buffer, 5X Reaction Buffer
• Quantity: 10,000 units
• Product Type: S1 Nuclease

For Research Use Only. Not for use in diagnostic procedures.