Thermo Scientific T3 RNA Polymerase
Catalyze the 5'→3' synthesis of RNA on either single-stranded or double-stranded DNA with T73 RNA polymerase.
Manufacturer: Thermo Scientific EP0101
Bacteriophage T3 RNA polymerase is DNA-dependent RNA polymerase with strict specificity for their respective double-stranded promoters. It catalyzes the 5'→3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter and is able to incorporate modified nucleotide.>/p>It catalyzes the 5'=>3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from its promoter.
- Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)
Synthesis of unlabeled and labeled RNA that can be used: for hybridization (1), in vitro RNA translation (2); as aRNA (3), siRNA (4), substrate in RNase protection assays (5), template for genomic DNA sequencing.
Consensus promoter sequences: AATTAACCCTCACTAAA G GGAGAT3 TAATACGACTCACTATA G GGAGAT7 ATTTAGGTGACACTATA G AAGNGSP6 The position in bold (+1) indicates the first nucleotide incorporated into RNA during transcription. Only bases at this position through +3 are critical for transcription, and they must be a G and a purine base, respectively (9).
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|Synthesis of unlabeled and labeled RNA that can be used: for hybridization (1), in vitro RNA translation (2); as aRNA (3), siRNA (4), substrate in RNase protection assays (5), template for genomic DNA sequencing.|
|T3 RNA Polymerase|
|1mL of 5X Transcription Buffer|
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