Thermo Scientific™ Taq DNA Polymerase, recombinant (5 U/μL)

Description

Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus.

Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5' to 3' synthesis of DNA, has no detectable 3' to 5' exonuclease (proofreading) activity, and possesses low 5' to 3' exonuclease activity. In addition, Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Recombinant Taq DNA Polymerase is the ideal tool for standard PCR of templates 5 kb or shorter.

Features

• Thermostable—half life is more than 40 min at 95°C
• Generates PCR products with 3'-dA overhangs
• Supplied with two buffers: 10X Taq Buffer with KCl and 10X Taq Buffer with (NH₄)₂SO₄
• Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescent-labeled nucleotides)

Applications

• Routine PCR amplification of DNA fragments up to 5 kb
• High throughput PCR
• DNA labeling

Notes

• The error rate of Taq DNA Polymerase in PCR is 2.2 x 10^-5 errors per nt per cycle. Accordingly, the accuracy of PCR is 4.5 x 10⁴. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.
• 10X Taq Buffer without Detergent is recommended for microarray experiments.
• 10X Taq Buffer with (NH₄)₂SO₄ allows for PCR at wide range of magnesium concentrations and decreases unspecific priming.

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Specifications

• Concentration: 5 U/μL
• Content And Storage: • Taq DNA Polymerase (5 U/μL), 5 x 100 μL
  • 10X Taq Buffer with KCl, 10 x 1.25 mL
  • 10X Taq Buffer with (NH₄)₂SO₄, 10 x 1.25 mL
  • 25 mM MgCl₂, 10 x 1.25 mL
  
  Store at -20°C.
• GC-Rich PCR Performance: Low
• Polymerase: Taq DNA Polymerase
• Reaction Speed: Standard
• Product Type: Taq DNA Polymerase (Recombinant)
• Quantity: 5 x 500 Units
• Shipping Condition: Dry Ice
• For Use With (Application): Standard PCR
• Fidelity (vs. Taq): 1X
• Hot Start: No
• Overhang: 3'-A
• Reaction Format: Separate Components

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.