Ferritin Polyclonal Antibody, Biotin, Rockland™

Rabbit Polyclonal Antibody

Supplier:  Rockland Immunochemicals 200406090S

Catalog No. 50-199-0977

Description

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Anti-Ferritin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Ferritin [Human Spleen]. Cross reactivity against Ferritin from other sources may occur but has not been specifically determined.

Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. Ferritin is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in the light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. The ferritin gene has multiple pseudogenes, and ferritin is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis. Ferritin is a holoenzyme shell with a molecular weight of about 450 kDa, and consists of 24 subunits of two types, H(heavy) and L(light), and capable of storing up to 4,500 atoms of ferric iron.

Specifications

• Antigen: Ferritin
• Classification: Polyclonal
• Conjugate: Biotin
• Gene: FTL
• Gene Alias: apoferritin; Cell proliferation-inducing gene 15 protein; Ferritin H subunit; Ferritin heavy chain; ferritin heavy chain 1; Ferritin heavy chain, N-terminally processed; Ferritin L subunit; ferritin L-chain; Ferritin light chain; ferritin light polypeptide-like 3; Ferritin subunit H; ferritin, heavy polypeptide 1; ferritin, light polypeptide; FHC; FTH; Fth1; FTHL6; FTL; HFE5; H-ferritin; HFt; L apoferritin; LFTD; MFH; MGC71996; NBIA3; OK/SW-cl.84; PIG15; placenta immunoregulatory factor; PLIF; proliferation-inducing protein 15
• Host Species: Rabbit
• Quantity: 25 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody

• Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
• Concentration: 1 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
• Gene Accession No.: P02792, P02794
• Gene Symbols: FTH1, FTL
• Immunogen: Ferritin [Human Spleen]
• Regulatory Status: RUO
• Gene ID (Entrez): 2495, 2512
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Lyophilized