Novus Biologicals™ Ficolin-1 Antibody Pair [HRP]

Description

Mouse Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human Ficolin-1.

Specifications

• Assay Range: 31.25 pg/mL
• Assay Sensitivity: 31.25-2000 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human Ficolin-1.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: Collagen/fibrinogen domain-containing protein 1, FCNMficolin-A, ficolin (collagen/fibrinogen domain containing) 1, ficolin (collagen/fibrinogen domain-containing) 1, ficolin-1, Ficolin-A, Ficolin-alpha, M-ficolin
• Gene ID (Entrez): 2219
• Gene Symbol: FCN1
• Host Species: Mouse
• Kit Contents:
  Standard: Each vial contains 38 ng of recombinant Human Ficolin-1.
  Capture Antibody: 0.3 mg/mL of mouse anti-Human Ficolin-1 monoclonal antibody (in PBS, pH 7.4).
  Detection Antibody: 0.5 mg/mL of mouse anti-Human Ficolin-1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
• Quantity: 15 Plates
• Regulatory Status: RUO
• Storage Requirements: Storage is content dependent.
• Target: Ficolin-1
• Technique: 1. Capture Antibody: Dilute to a working concentration of 1 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.1 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2000 pg/mL is recommended.