Cytiva Ficoll PM400

High molecular weight sucrose-polymers formed by copolymerization of sucrose with epichlorohydrin. Cytiva™ Ficoll PM400 is a synthetic neutral, highly-branched hydrophilic polymer of sucrose, used for producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity.

Supplier:  Cytiva 17030005

Catalog No. 45-001-792

Description

• For producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity
• Neutral, highly branched, hydrophilic polymer of sucrose which dissolves readily in aqueous solution
• Concentrations of up to 50% (w/v) covering densities of up to 1.2g/mL can be achieved
• Has better osmotic properties than sucrose
• Preserves functional and morphological integrity
• Useful for separating cells that are sensitive to centrifugation and for separating cells of similar density but different size
• Does not penetrate biological membranes
• Serves as the raw material for preparation of Ficoll-Paque gradients
• Used in other applications such as electrophoresis, hybridization, cryopreservation, and as a hapten carrier
• Supplied as dry powders

• Used in the formation of density gradients for the separation and isolation of eukaryotic cells, organelles, and bacterial cells, as a stabilizing agent and as a separation medium for the isolation of lymphocytes
• Used in defined culture media, nucleic acid hybridization, electrophoresis, and immunological studies
• Used for gradient centrifugation in all types of centrifuge rotors and for separation at unit gravity

Specifications

• Quantity: 5 kg
• For Use With (Application): Cell Isolation
• Endotoxin Level: 0.12 EU/mg
• Product Type: Centrifugation Media
• Form: Powder

• Content And Storage: 4–30°C
• Viscosity: 5 ηr at 20°C: 10% (w/v), 20 ηr at 20°C: 20% (w/v), 60 ηr at 20°C : 30% (w/v, 180 ηr at 20°C : 40% (w/v) , 600 ηr at 20°C : 50% (w/v)
• Color: White
• Density: 1.2 g/mL

Safety and Handling

ShelfLife : 5 years