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Cytiva Ficoll PM400

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Quantity:
100 g
500 g
5 kg
Description
- For producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity
- Neutral, highly branched, hydrophilic polymer of sucrose which dissolves readily in aqueous solution
- Concentrations of up to 50% (w/v) covering densities of up to 1.2g/mL can be achieved
- Has better osmotic properties than sucrose
- Preserves functional and morphological integrity
- Useful for separating cells that are sensitive to centrifugation and for separating cells of similar density but different size
- Does not penetrate biological membranes
- Serves as the raw material for preparation of Ficoll-Paque gradients
- Used in other applications such as electrophoresis, hybridization, cryopreservation, and as a hapten carrier
- Supplied as dry powders
- Used in the formation of density gradients for the separation and isolation of eukaryotic cells, organelles, and bacterial cells, as a stabilizing agent and as a separation medium for the isolation of lymphocytes
- Used in defined culture media, nucleic acid hybridization, electrophoresis, and immunological studies
- Used for gradient centrifugation in all types of centrifuge rotors and for separation at unit gravity
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Specifications
Specifications
Quantity | 5 kg |
Content And Storage | 4–30°C |
For Use With (Application) | Cell Isolation |
Viscosity | 5 ηr at 20°C: 10% (w/v), 20 ηr at 20°C: 20% (w/v), 60 ηr at 20°C : 30% (w/v, 180 ηr at 20°C : 40% (w/v) , 600 ηr at 20°C : 50% (w/v) |
Endotoxin Level | 0.12 EU/mg |
Color | White |
Product Type | Centrifugation Media |
Density | 1.2 g/mL |
Form | Powder |
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