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Invitrogen™ Firefly luciferase Monoclonal Antibody (LUC-Y)
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Catalog No. PIMA180225
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100 μg
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PIMA180225 100 μg
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Catalog No. PIMA180225 Supplier Invitrogen™ Supplier No. MA180225
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Description

Mouse Monoclonal Antibody

Membrane permeabilization is required for flow cytometry applications. In Western Blot , MA1-80225 detects a band at ~55-60 kDa using 1 μg of purified luciferase. For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Mouse anti Luciferase antibody, clone LUC-Y detects the expression of the luciferase gene of the North American firefly Photinus pyralis in cells and tissues.

Luciferase produces green light with the wavelength of 562 nm. Luciferase catalyzes the reaction: D-firefly luciferin + O2 + ATP = firefly oxyluciferin + CO2 + AMP + diphosphate + light.
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Specifications

Antigen Firefly luciferase
Applications ELISA, Flow Cytometry, Immunohistochemistry (Frozen), Western Blot, Immunocytochemistry
Classification Monoclonal
Clone LUC-Y
Concentration 1 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.09% sodium azide
Gene Alias Firefly Luciferase; Genji firefly; Green Luciferase; Japanese firefly; luciferin 4-monooxygenase; Photinus pyralis; photinus pyralis luciferase
Host Species Mouse
Immunogen Recombinant luciferase expressed in E. Coli.
Purification Method Protein A
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Luciferase
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Type Antibody
Form Liquid
Isotype IgG1
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