Invitrogen™ Firefly luciferase Monoclonal Antibody (LUC-Y)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA180225

Catalog No. PIMA180225

Description

Membrane permeabilization is required for flow cytometry applications. In Western Blot , MA1-80225 detects a band at ~55-60 kDa using 1 μg of purified luciferase. For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Mouse anti Luciferase antibody, clone LUC-Y detects the expression of the luciferase gene of the North American firefly Photinus pyralis in cells and tissues.

Luciferase produces green light with the wavelength of 562 nm. Luciferase catalyzes the reaction: D-firefly luciferin + O2 + ATP = firefly oxyluciferin + CO2 + AMP + diphosphate + light.

Specifications

• Antigen: Firefly luciferase
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.09% sodium azide
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Target Species: Luciferase
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Frozen), Western Blot, Immunocytochemistry
• Clone: LUC-Y
• Conjugate: Unconjugated
• Gene Alias: Firefly Luciferase; Genji firefly; Green Luciferase; Japanese firefly; luciferin 4-monooxygenase; Photinus pyralis; photinus pyralis luciferase
• Immunogen: Recombinant luciferase expressed in E. Coli.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid