Cytiva First-Strand cDNA Synthesis Kit

Description

Includes

Bulk first-strand cDNA reaction mixes (Cloned murine reverse transcriptase, RNase inhibitor, RNase/DNase-free BSA, dATP, dCTP, dGTP, and dTTP in aqueous buffer), mRNA standard, DTT solution, RNase-free water, pd(N)₆ primer, and Not I-(dT)₁₉ bifunctional primer

For synthesis of single-stranded cDNA from mRNA templates prior to amplification by PCR

• Preassembled bulk reaction mixes save time and minimize potential for pipetting errors
• Primers are not included in the preassembled reaction mixes, allowing use of a primer of choice
• Two primers are included with the kit
• pd(N)₆ primer (random hexamer) is used to prepare libraries for screening with antibodies, to increase the representation of 5'-ends of mRNAs having significant secondary structure, or to copy mRNAs lacking a poly(A) tail
• Not I-(dT)₁₈ bifunctional primer can be used to prime selectively on mRNA having a poly(A) tail and can also be used as a PCR primer following first-strand synthesis
• The Not I restriction site, located at the 5'-end of the primer, is particularly useful for subsequent directional cloning of cDNA and PCR products

Specifications

• Content And Storage: -15°C to -30°C
• Description: Designed to generate full-length first-strand cDNA from mRNA templates. Following first-strand cDNA synthesis, the sample can be used directly for in vitro amplification using PCR or for second-strand synthesis using the Gubler-Hoffman method.
• For Use With (Application): RT-PCR
• Includes: Bulk first strand cDNA reaction mixes, mRNA standard, DTT solution, RNase-free Water, pd(N)6 primer and NotI-(dT)18 bifunctional primer
• No. of Reactions: 55
• Product Type: First-Strand cDNA Synthesis Kit
• Quantity: 55 Reactions