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Molecular Probes™ FISH Tag™ DNA Kits with Alexa Fluor™ Dyes
Description
Choose from three, single-color FISH Tag DNA kits with Alexa Fluor dye options (green, orange, or red). The FISH DNA multicolor kit contains four spectrally distinct dyes (green, orange, red, and deep-red) to allow simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and in situ analysis of mitochondrial DNA localization and content. All kits include 16 reagents and come with a comprehensive, detailed protocol to help ensure your success.
FISH Tag™ DNA Kits provide a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. Choose from FISH Tag™ DNA Kits in three Alexa Fluor™ colors or get the FISH Tag™ DNA Multicolor Kit (Cat. No. F32951), which contains four spectrally distinct dyes to allow simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and for in situ analysis of mitochondrial DNA localization and content. All kits include 16 reagents and come with a comprehensive, detailed protocol to help ensure your success.
FISH Tag™ Kit Specifications:
- Dye (Ex/Em): Alexa Fluor™ 594 (590/615 nm)
- Comprehensive kit includes 16 reagents
- Comes with detailed protocol to help ensure your success
Complete Workflow Solution for FISH
FISH Tag™ Kits employ a two-step labeling technology—nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor™ dyes. PureLink™ nucleic acid purification technology is used for fast and efficient purification of the labeled probe, and SlowFade™ Gold antifade reagent is added to preserve the fluorescent signal during imaging.
More Options for Nucleic Acid Labeling
To review various options for nucleic acid labeling (including DNA and RNA FISH), review Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes™ Handbook.
For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
Specifications
Specifications
| Content And Storage | Store in freezer (-5 to -30°C) and protect from light. |
| Color | Green |
| Detection Method | Fluorescence |
| Format | Kit |
| Label or Dye | Alexa Fluor 488 |
| Final Product Type | Probes (Labeled DNA), cDNA (Labeled) |
| Sample Type | DNA, Tissue (FFPE), Tissue (CRYO), Cell Culture Cells |
| Labeling Target | DNA (General), cDNA |
| Excitation/Emission | 492/520 nm |
| For Use With (Application) | FISH Tag, fluorescent microscopy, DNA In situ hybridization |
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Frequently Asked Questions (FAQs)
FISH probes are generally from 300 to 1500 bp in length. To adequately image a gene or other target, the FISH probes must cover a contiguous length of ˜10 kbp. Shorter strand lengths or low-abundance DNA or RNA targets may be detected with the use of fluorescent amplification methods, such as tyramide signal amplification (TSA). For more information using TSA to amplify FISH detection, see this manual (http://tools.thermofisher.com/content/sfs/manuals/mp20912.pdf).
RNA FISH probes are the best option for hybridization to mRNA, rRNA, or other RNA targets. DNA FISH probes can also be used to detect RNA targets, but they do not hybridize as well to mRNA and other RNA target molecules. DNA FISH probes are routinely used to detect gene loci on chromosomes. RNA FISH probes can also be used to detect genes on chromosomes, but DNA FISH probes are more robust for this application.
For Research Use Only. Not for use in diagnostic procedures.
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