Fisher BioReagents™ Optizyme™ Ribonuclease Inhibitor, Recombinant

Description

Protects RNA from degradation by RNases

• Supplied as a recombinant product from E. coli (originally isolated from rat lung) and provides superior protection of RNA from degradation by RNases
• Commonly added to all solutions used during the isolation of RNA, and will not interfere with the performance of most enzymes in downstream applications
• Active over a broad temperature range, and even provides some RNase inhibition at 60°C, which is useful when performing reverse transcription reactions at elevated temperatures to overcome secondary structure in RNA
• Storage Buffer Components: 20mM HEPES-KOH (pH 7.6 at 4°C), 50mM KCl, 8mM DTT, and 50% glycerol (v/v)

Inactivates RNase A, RNase B, and RNase T2; No activity against SP6, T7, or T3 RNA polymerases, AMV or MMLV reverse transcriptases, and Taq DNA polymerase

• Displays a broad spectrum of inhibitory activity against RNases, and does not have activity against other polymerases and reverse transcriptases
• Suitable for use in common molecular biology applications such as isolation and purification of RNA, cDNA synthesis, RT-PCR, in vitro RNA transcription/translation, ribonuclease protection assay, and preparation of RNase-free antibodies

Specifications

• Concentration: 20 to 40 U/μL
• Color: Colorless
• For Use With (Application): Isolation and purification of RNA, cDNA synthesis, RT-PCR, in-vitro RNA transcription/translation, Ribonuclease protection assay and preparation of RNase-free Antibodies
• Storage Buffer: 20mM HEPES-KOH (pH 7.6 at 4°C), 50mM KCl, 8mM DTT, 50% Glycerol
• Source: E. coli
• Quantity: 2500 U
• Product Type: RNase Inhibitor
• Form: Liquid

Safety and Handling

SDS