Invitrogen™ FITC Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 711900

Catalog No. 71-190-0

Description

This antibody was purified from serum of rabbit hyper-immunized with FITC conjugated to KLH. The antibody was purified by antigen-specific matrix. The antibody reacts with FITC but not the carrier protein. Useful as a secondary antibody in a wide range of immunoassays including: ELISA, tissue and cell staining, immunoprecipitation, and western blotting. The protein concentration is determined based on an E (1%) = 14 at 280 nm.

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Anti-FITC antibodies can be used for isolating FITC labeled cells by magnetic microbeads based sorting, as well as removal of background signal.

Specifications

• Antigen: FITC
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene Alias: Fluorescein
• Immunogen: FITC.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: -20°C
• Form: Liquid
• Target Molecule: Chemical

• Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
• Concentration: 0.25 mg/mL
• Formulation: PBS with 0.1% sodium azide; pH 7.4
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Target Species: Chemical
• Product Type: Antibody
• Isotype: IgG